Generation of protein carbonyls by glycoxidation and lipoxidation reactions with autoxidation products of ascorbic acid and polyunsaturated fatty acids
Autor: | Koichi Asahi, Reiko Inagi, Toshio Miyata, Koji Uchida, Yukihiro Yamada, Kiyoshi Kurokawa, Hideto Sakai, Katsunori Horie |
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Jazyk: | angličtina |
Předmět: |
Glycation End Products
Advanced Aging Glycosylation Arteriosclerosis Lipoproteins Protein Carbonylation Glycoxidation Biophysics Ascorbic Acid Arginine Biochemistry chemistry.chemical_compound Structural Biology Glycation Malondialdehyde Genetics Humans Pentosidine Molecular Biology chemistry.chemical_classification Arachidonic Acid Autoxidation Lysine Cell Biology Ascorbic acid Atherosclerosis Lipoxidation Oxidative Stress Glucose chemistry Advanced glycation end-product Lipid Peroxidation Protein carbonyl Advanced glycation end product Oxidation-Reduction Diabetic Angiopathies Polyunsaturated fatty acid |
Zdroj: | FEBS Letters. (1-2):24-28 |
ISSN: | 0014-5793 |
DOI: | 10.1016/S0014-5793(98)01079-5 |
Popis: | Accumulation of carbonyl derivatives of proteins (protein carbonyl) is taken as a biomarker of oxidative protein damage in aging and in various diseases. We detected protein carbonyls in situ in human diabetic arteriosclerotic tissues and characterized the formation of protein carbonyls. Protein carbonyls were identified in the thickened intima of arterial walls and co-localized with protein adducts formed by carbonyl amine chemistry between protein and carbonyl compounds derived from autoxidation of carbohydrates, lipids, and ascorbate, i.e. advanced glycation end products or glycoxidation products, such as carboxymethyllysine (CML) and pentosidine, and lipoxidation products, such as malondialdehyde (MDA) and 4-hydroxy-nonenal (HNE). In vitro incubation of proteins with ascorbic acid accelerated the production of protein carbonyls as well as CML and pentosidine, and incubation with arachidonate accelerated the production of protein carbonyls as well as CML, MDA, and HNE. By contrast, incubation of proteins with glucose resulted in the production of CML and pentosidine, but not protein carbonyls. Schiff base inhibitors, (±)-2-isopropylidenehydrazono-4-oxo-thiazolidin-5-ylacetanilide and aminoguanidine, inhibited the production of protein carbonyls after incubation with ascorbate and arachidonate. The present study suggests that ascorbate and polyunsaturated fatty acids, but not glucose, represent potential sources of protein carbonyls, and that both the glycoxidation and lipoxidation reactions contribute to protein carbonyl formation in aging and various diseases. |
Databáze: | OpenAIRE |
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