miRNA Stability in Frozen Plasma Samples
| Autor: | Luca Deiana, Sara Pasella, Baingio Porcu, Angela Baralla, Marta Deiana, Ciriaco Carru, Giuseppe Fanciulli, Annalisa Oggiano, Andrea Mannu, Mario Pescatori, Silvia Dei Giudici, Francesca Balzano, Angelo Zinellu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
RNA Stability Pharmaceutical Science Repressor Biology Article Cryopreservation Analytical Chemistry lcsh:QD241-441 lcsh:Organic chemistry Drug Discovery microRNA sequences AU or UA Humans Physical and Theoretical Chemistry Gene miRNA Gene Expression Profiling Organic Chemistry Half-life Middle Aged stability Molecular biology Healthy Volunteers Gene expression profiling MicroRNAs Biochemistry Blood Preservation Chemistry (miscellaneous) Molecular Medicine Fresh frozen plasma long freezing times Half-Life |
| Zdroj: | Molecules Volume 20 Issue 10 Pages 19030-19040 Molecules, Vol 20, Iss 10, Pp 19030-19040 (2015) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules201019030 |
| Popis: | MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life. |
| Databáze: | OpenAIRE |
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