Production of humanized F(ab’)2 fragment of rabies blocking antibodies in Pichia pastoris yeast
| Autor: | O. N. Solopova, S. S. Zatsepin, S. V. Benevolensky, O. B. Shemchukova, P. G. Sveshnikov, T A Yagudin, L. P. Pozdnyakova, E V Klyatchko, E. V. Morozkina |
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| Předmět: |
0301 basic medicine
biology Immunoglobulin Fab Fragments Rabies virus biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Molecular biology Yeast law.invention Pichia pastoris 03 medical and health sciences 030104 developmental biology law Chaperone (protein) Blocking antibody Recombinant DNA medicine biology.protein Pichia |
| Zdroj: | Scopus-Elsevier |
| Popis: | The yeast strain Pichia pastoris, a producer of humanized F(ab’)2 fragments of rabies-blocking antibodies, has been obtained. Human chaperone BiP coexpression caused a twofold increase of the immunoglobulins secretion level. The use of Fos and Jun zippers in the composition of heavy chains facilitated the dimerization of F(ab’)2 fragments of the shared pool of secreted immunoglobulins up to 75%. |
| Databáze: | OpenAIRE |
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