The mouse Hox-1.3 gene is functionally equivalent to the Drosophila Sex combs reduced gene
| Autor: | Leslie Pick, J. J. Zhao, R. A. Lazzarini |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Embryo
Nonmammalian Hot Temperature animal structures Recombinant Fusion Proteins Molecular Sequence Data Restriction Mapping Pair-rule gene Gene Expression Biology behavioral disciplines and activities Animals Genetically Modified Fusion gene Mice Open Reading Frames Transformation Genetic Drosophilidae Gene expression Genetics Animals Amino Acid Sequence Hox gene Gene Heat-Shock Proteins Base Sequence Sequence Homology Amino Acid fungi Genes Homeobox biology.organism_classification Phenotype Gene Expression Regulation Oligodeoxyribonucleotides Larva Drosophila Ectopic expression Homeotic gene Developmental Biology |
| Zdroj: | Genes & Development. 7:343-354 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.7.3.343 |
| Popis: | To test whether the mouse Hox-1.3 gene is a cognate of the Drosophila Sex combs reduced (Scr) gene, we inserted a hsp 70-Hox-1.3 fusion gene into the Drosophila genome. Transgenic flies displayed Scr-like homeotic transformations after ectopic expression of Hox-1.3 induced by heat shock. In larvae, the thoracic segments T2 and T3 are transformed toward T1. In adults, head structures are dramatically disrupted, including transformation of antenna towards leg. Transformations are not the result of ectopic activation of the endogenous Scr gene. Rather, Hox-1.3 appears to directly regulate Scr target genes, as demonstrated by the ectopic activation of fork head by Hox-1.3. The results suggest that mouse Hox-1.3 cannot only substitute functionally for Drosophila Scr in the determination of external structures but also can participate in the regulatory hierarchy of insect organogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|