Alteration in the density, morphology, and biological properties of eosinophils produced by bullous pemphigoid blister fluid
| Autor: | Mamoru Kasada, Shingo Tsuda, Kayoko Iryo, Yoichiro Sasai, Minoru Miyasato |
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| Rok vydání: | 1989 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Count Receptors Cell Surface Dermatology Biology Granulocyte chemistry.chemical_compound Cell surface receptor Pemphigoid Bullous Centrifugation Density Gradient medicine Animals Humans skin and connective tissue diseases Receptor Differential centrifugation Skin Diseases Vesiculobullous integumentary system Cell Membrane Zymosan General Medicine Eosinophil medicine.disease biology.organism_classification Molecular biology Body Fluids Eosinophils Microscopy Electron medicine.anatomical_structure chemistry Luminescent Measurements Schistosoma Bullous pemphigoid Schistosoma mansoni |
| Zdroj: | Archives of Dermatological Research. 281:304-309 |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/bf00412972 |
| Popis: | Bullous pemphigoid blister fluid (BP-BF) was examined for its effects on the density, morphology, and biological properties of eosinophils. Normodense eosinophils (NEo) were prepared from guinea pig peritoneal exudates by Nycodenz density gradient centrifugation. After culturing with BP-BF, NEo were converted into hypodense eosinophils (HEo) in a time-dependent manner. HEo were morphologically different from NEo in that HEo had spheroidal granules each with a lytic crystalloid core and a significantly increased cell volume. These HEo showed an enhanced antibody- and/or complement-dependent helminthotoxic activity to Schistosoma mansoni larvae, amplified chemiluminescence response to opsonized zymosan, and augmented expression of both FcR+ and CR+. These results suggest that BP-BF contains an activity that may not only induce an eosinophil hypodensity as a consequence of increasing cell volume, but simultaneously enhance an eosinophil cytotoxic potential through augmenting cell-surface receptors and receptor-linked oxidative metabolism. In addition, observed tissue accumulation of this activity suggests that eosinophils may be regulated by their phenotypic change in the skin lesions of bullous pemphigoid and be involved in blister formation. |
| Databáze: | OpenAIRE |
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