Cimetidine‐induced androgenic failure causes cell death and changes in actin, EGF and V‐ATPase immunoexpression in rat submandibular glands
| Autor: | Estela Sasso-Cerri, Fabiane de Santi, Mariane Castro Manzato, Flávia L. Beltrame, Paulo Sérgio Cerri, André Acácio Souza da Silva |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Vacuolar Proton-Translocating ATPases Histology Ductal cells Cytochrome P-450 CYP1A2 Inhibitors salivary glands Submandibular Gland Drug Evaluation Preclinical Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine stomatognathic system Internal medicine medicine Acinar cell Animals Testosterone Cimetidine Molecular Biology Ecology Evolution Behavior and Systematics Epidermal Growth Factor Chemistry apoptosis Cell Biology Submandibular gland Original Papers Actins Androgen receptor 030104 developmental biology medicine.anatomical_structure Endocrinology Cytoplasm Apoptosis Receptors Androgen testosterone antiandrogen Anatomy 030217 neurology & neurosurgery AR Developmental Biology medicine.drug |
| Zdroj: | J Anat Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T10:25:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Submandibular gland (SMG) is responsive to androgens via androgen receptor (AR). We verified whether cimetidine induces androgenic dysfunction in SMG, and evaluated the structural integrity, cell death and immunoexpression of actin, EGF and V-ATPase in androgen-deficient SMG. Male rats received cimetidine (CMTG) and control animals (CG) received saline. Granular convoluted tubules (GCTs) diameter and number of acinar cell nuclei were evaluated. TUNEL and immunofluorescence reactions for detection of AR, testosterone, actin, EGF and V-ATPase were quantitatively analysed. In CG, testosterone immunolabelling was detected in acinar and ductal cells cytoplasm. AR-immunolabelled nuclei were observed in acinar cells whereas ductal cells showed AR-immunostained cytoplasm, indicating a non-genomic AR action. In CMTG, the weak testosterone and AR immunoexpression confirmed cimetidine-induced androgenic failure. A high cell death index was correlated with decreased number of acinar cells, GCTs diameter and EGF immunoexpression under androgenic dysfunction. Actin immunofluorescence decreased in the SMG cells, but an increased and diffuse cytoplasmic V-ATPase immunolabelling was observed in striated ducts, suggesting a disruption in the actin-dependent V-ATPase recycling due to androgenic failure. Our findings reinforce the androgenic role in the maintenance of SMG histophysiology, and point to a potential clinical use of cimetidine against androgen-dependent glandular tumour cells. Department of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (Unesp) Department of Morphology and Genetics Federal University of São Paulo Department of Morphology Genetics Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (Unesp) |
| Databáze: | OpenAIRE |
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