Simultaneous quantification of α-/β-diastereomers of arteether, sulphadoxine and pyrimethamine: A promising anti-relapse antimalarial therapeutic combination, by liquid chromatography tandem mass spectrometry☆
| Autor: | Ram Chandra Gupta, S Sabarinath, Ravi Shankar Prasad Singh |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Electrospray ionization Clinical Biochemistry Ethyl acetate Tandem mass spectrometry Mass spectrometry Sensitivity and Specificity Biochemistry High-performance liquid chromatography Analytical Chemistry Antimalarials chemistry.chemical_compound Liquid chromatography–mass spectrometry Sulfadoxine Secondary Prevention Animals Chromatography High Pressure Liquid Antibacterial agent Chromatography Stereoisomerism Cell Biology General Medicine Reference Standards Artemisinins Malaria Rats Pyrimethamine chemistry Ammonium acetate |
| Zdroj: | Journal of Chromatography B. 842:36-42 |
| ISSN: | 1570-0232 |
| Popis: | A rapid, sensitive, selective and specific HPLC/ESI-MS/MS assay method was developed and validated for the simultaneous quantitation of alpha-/beta-diastereomers of arteether (AE), sulphadoxine (SDX) and pyrimethamine (PYR) in rat blood plasma using propyl ether analogue of beta-arteether as internal standard. The method involved a single-step, liquid-liquid extraction with ethyl acetate and the analytes were chromatographed on a C18 chromatographic column by isocratic elution with methanol:ammonium acetate buffer (10 mM, pH 4) (90:10%, v/v) and analyzed by tandem mass spectrometry. The run time was 4.5 min and the weighted (1/x2) calibration curves were linear over a range of 0.78-400 ng ml-1. The method was validated fully and the lower limit of quantification (LLOQ) in plasma was 0.78 ng ml-1 for all the analytes. The intra- and inter-day precision and accuracy were found to be well within the acceptable limits ( |
| Databáze: | OpenAIRE |
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