Debugging diversity - a pan-continental exploration of the potential of terrestrial blood-feeding leeches as a vertebrate monitoring tool
| Autor: | Mikkel-Holger S. Sinding, Andrew Tilker, Anh Nguyen Quang Hoa, Adam Frederik Sander Pedersen, Ida Bærholm Schnell, Douglas W. Yu, Sigrid Heise-Pavlov, Sebastian E. Schultze, Nicholas M. Wilkinson, Thomas N. E. Gray, Dáithí C. Murray, Mark E. Siddall, M. Thomas P. Gilbert, Michael Bunce, Juliot Carl Ramamonjisoa, Rainer Dolch, Paul Woodcock, Kristine Bohmann, Stine Raith Richter, David Bass, Mason J. Campbell, Teis Hansen, Carl Traeholt, Mads F. Bertelsen, Christina Lehmkuhl Noer, David Edwards, John E. Cadle |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Range (biology) Biodiversity Biology 010603 evolutionary biology 01 natural sciences DNA barcoding Amphibians Birds 03 medical and health sciences Leeches biology.animal Genetics Animals DNA Barcoding Taxonomic Environmental DNA Ecology Evolution Behavior and Systematics Invertebrate Mammals Ecology High-Throughput Nucleotide Sequencing Reptiles Vertebrate 030104 developmental biology Taxon Metagenomics Blood Chemical Analysis Environmental Monitoring Biotechnology |
| Popis: | The use of environmental DNA (eDNA) has become an applicable non‐invasive tool with which to obtain information about biodiversity. A sub‐discipline of eDNA is iDNA (invertebrate‐derived DNA), where genetic material ingested by invertebrates is used to characterise the biodiversity of the species that served as hosts. While promising, these techniques are still in their infancy, as they have only been explored on limited numbers of samples from only a single or a few different locations. In this study, we investigate the suitability of iDNA extracted from more than 3,000 haematophagous terrestrial leeches as a tool for detecting a wide range of terrestrial vertebrates across five different geographical regions on three different continents. These regions cover almost the full geographical range of haematophagous terrestrial leeches, thus representing all parts of the world where this method might apply. We identify host taxa through metabarcoding coupled with high‐throughput sequencing on Illumina and IonTorrent sequencing platforms to decrease economic costs and workload and thereby make the approach attractive for practitioners in conservation management. We identified hosts in four different taxonomic vertebrate classes: mammals, birds, reptiles, and amphibians, belonging to at least 42 different taxonomic families. We find that vertebrate blood ingested by haematophagous terrestrial leeches throughout their distribution is a viable source of DNA with which to examine a wide range of vertebrates. Thus, this study provides encouraging support for the potential of haematophagous terrestrial leeches as a tool for detecting and monitoring terrestrial vertebrate biodiversity. |
| Databáze: | OpenAIRE |
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