Deorphanization of novel biogenic amine-gated ion channels identifies a new serotonin receptor for learning
| Autor: | Iris Hardege, Taihong Wu, He Liu, Julia Morud, Yun Zhang, Myung-Kyu Choi, William R Schafer, Swaraj Basu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biogenic Amines
ligand-gated ion channels Olfaction Neurotransmission Biology Inhibitory postsynaptic potential tyramine General Biochemistry Genetics and Molecular Biology Ion Channels Postsynaptic potential Animals Caenorhabditis elegans Caenorhabditis elegans Proteins 5-HT receptor Ion channel learning serotonin Receptors Serotonin Excitatory postsynaptic potential C. elegans behavioural plasticity Ligand-gated ion channel dopamine General Agricultural and Biological Sciences Neuroscience olfaction |
| Popis: | Pentameric ligand-gated ion channels (LGICs) play conserved, critical roles in both excitatory and inhibitory synaptic transmission and can be activated by diverse neurochemical ligands. We have performed a characterization of orphan channels from the nematode C. elegans, identifying five new monoamine-gated LGICs with diverse functional properties and expression postsynaptic to aminergic neurons. These include polymodal anion channels activated by both dopamine and tyramine, which may mediate inhibitory transmission by both molecules in vivo. Intriguingly, we also find that a novel serotonin-gated cation channel, LGC-50, is essential for aversive olfactory learning of pathogenic bacteria, a process known to depend on serotonergic neurotransmission. Remarkably, the redistribution of LGC-50 to neuronal processes is modulated by olfactory conditioning, and lgc-50 point mutations that cause misregulation of receptor membrane expression interfere with olfactory learning. Thus, the intracellular trafficking and localization of these receptors at synapses may represent a molecular cornerstone of the learning mechanism. ispartof: CURRENT BIOLOGY vol:31 issue:19 pages:4282-+ ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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