Confirmation that the Novel Cercozoa Phytocercomonas venanatans Is the Cause of the Disease Chlorotic Streak in Sugarcane
| Autor: | Chuong Nguyen Ngo, Barry J. Croft, Kathryn Susan Braithwaite |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Streak Plant Science Disease Biology Plant Roots Polymerase Chain Reaction 01 natural sciences law.invention 03 medical and health sciences law Cercozoa Pathogen Gene Polymerase chain reaction Plant Diseases Plant Stems Pathogenicity biology.organism_classification Virology Saccharum Plant Leaves 030104 developmental biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 108:487-494 |
| ISSN: | 1943-7684 0031-949X |
| Popis: | A cercomonad, named Phytocercomonas venanatans, is confirmed as the cause of the sugarcane disease chlorotic streak. This was achieved by establishing aseptic liquid cultures of the pathogen isolated from internal pieces of sugarcane stalk tissue. Actively motile cultures of the pathogen were inoculated into sugarcane roots, stalks, and leaf whorls. Infected plants subsequently developed the characteristic symptoms of chlorotic streak. Infection was confirmed by PCR screening of plant tissues and by reisolation of the pathogen into aseptic culture followed by PCR and microscopic confirmation. P. venanatans is the first reported pathogenic cercomonad able to systemically infect higher plants and the first plant pathogenic cercozoan able to be successfully grown in axenic culture on common microbiological media. |
| Databáze: | OpenAIRE |
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