Novel Promoter Sequence Required for Inductive Expression of theAspergillus nidulansEndoglucanase GeneeglA
| Autor: | Kengo Shirai, Misako Yokoyama, Naoki Kato, Masashi Kato, Yoshikazu Endo, Tetsuo Kobayashi, Munenori Morimoto, Go Chikamatsu, Norihiro Tsukagoshi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Molecular Sequence Data
Mutagenesis (molecular biology technique) Cellobiose Cellulase Applied Microbiology and Biotechnology Biochemistry Aspergillus nidulans Analytical Chemistry chemistry.chemical_compound Upstream activating sequence Enzyme inducer Cellulose DNA Fungal Promoter Regions Genetic Molecular Biology Gene DNA Primers Genetics Base Sequence biology Organic Chemistry General Medicine biology.organism_classification Cell biology chemistry Enzyme Induction Mutagenesis Site-Directed biology.protein Xylans DNA Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 72:312-320 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.70278 |
| Popis: | Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning -153 to -138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|