Localization and quantitation of hyaluronan and sulfated glycosaminoglycans in the tissues and intraluminal fluid of the pig oviduct
| Autor: | H. Pertoft, L. Kjellén, Heriberto Rodriguez-Martinez, K. Suzuki, Paisan Tienthai |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty animal structures Syndecans Cell Survival Swine media_common.quotation_subject Reproductive technology Biology Glycosaminoglycan Endocrinology Capacitation Internal medicine Genetics medicine Animals Uterotubal junction Hyaluronic Acid Ampulla Molecular Biology Ovulation Fallopian Tubes media_common Glycosaminoglycans Membrane Glycoproteins urogenital system Histocytochemistry Sperm Spermatozoa Body Fluids Reproductive Medicine Oviduct Animal Science and Zoology Female Proteoglycans Heparitin Sulfate Sperm Capacitation Heparan Sulfate Proteoglycans Developmental Biology Biotechnology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1031-3613 |
| Popis: | Glycosaminoglycans (GAGs), hyaluronan (HA) and heparan sulfate (HS) were localized in the pre- and post-ovulatory oviducts of inseminated and control (non-inseminated) sows using biotinylated HA-binding protein (HABP) and anti-syndecan antibodies respectively. In addition, the concentrations of HA and total sulfated GAGs (S-GAGs) were measured in fluid collected in vivo from either a selected tubal segment (isthmus or ampulla) or from the contralateral whole oviduct (WO) of non-inseminated sows during proestrus–metoestrus. HA was localized in the lamina propria of the entire oviduct, but epithelial HA-labelling was only present in the sperm reservoir (utero-tubal junction–adjacent isthmus) in control and inseminated sows. In contrast, immunolabelling for HS proteoglycans (HSPGs, syndecans) was present on the entire epithelial lining, both pre and post ovulation and in both sow groups. Both HA and S-GAGs could be detected in the intraluminal fluid. Concentrations varied among sows and segments; those of the S-GAGs being higher (P |
| Databáze: | OpenAIRE |
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