Occurrence and characterisation of Rhizoctonia species causing diseases on ornamental plants in Italy
| Autor: | Pietro T. Formica, Mitsuro Hyakumachi, Giancarlo Polizzi, Dalia Aiello, Vladimiro Guarnaccia |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Ornamental nurseries Plant Science rDNA sequence homology Horticulture Biology Root and foliar symptoms Rhizoctonia 01 natural sciences Rhizoctonia solani 03 medical and health sciences Cutting Ornamental plant Botany Pathogenicity Internal transcribed spacer Ribosomal DNA Host (biology) food and beverages 030108 mycology & parasitology biology.organism_classification Ornamental nurseries Pathogenicity Root and foliar symptoms rDNA sequence homology Agronomy and Crop Science 010606 plant biology & botany |
| Popis: | During surveys conducted in 2010–2012 Rhizoctonia symptoms were observed on 30 ornamental species in different nurseries located in eastern Sicily (Southern Italy). Eighty-eight isolates of Rhizoctonia spp. were obtained from symptomatic leaves, roots and stems. Fifty-six of the isolates were binucleate and 32 were multinucleate Rhizoctonia. Characterisation of anastomosis groups (AGs) was performed using morphological characteristics and sequence analysis of the internal transcribed spacer of ribosomal DNA ( rDNA-ITS) region. Most isolates collected were Rhizoctonia solani AG-4 HG-I (35.2% of all isolates) and one isolate was AG-2-2 IIIB. The binucleate isolates belonged to AG-R (27.3%), AG-A (21.6%), AG-G (12.5%), AG-V (1.1%) and AG-Fb (1.1%). The pathogenicity of 38 representative isolates collected from each host was tested on seedlings or cuttings grown in a growth chamber. All R. solani AG-4 HG-I isolates, most of the binucleate AG-R, AG-A and AG-G and AG-V were pathogenic and reproduced symptoms identical to that observed in nurseries, while binucleate AG-Fb and R. solani AG-2-2 IIIB isolates were nonpathogenic. This is the first report of the occurrence of Rhizoctonia species on some ornamental plants and the first report of binucleate Rhizoctonia AG-R and AG-V in Europe. |
| Databáze: | OpenAIRE |
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