The effects of gonadotrophin releasing hormone analogues in prostate cancer are mediated through specific tumour receptors
| Autor: | Jonathan Waxman, William J. Gullick, K. Sikora, R. C. Clayton, A. Qayum |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
endocrine system Cancer Research medicine.medical_specialty Radioimmunoassay Biology Buserelin Cell Line Prostate cancer DU145 Prostate Internal medicine LNCaP medicine Humans Oncology & Carcinogenesis Receptor Science & Technology Dose-Response Relationship Drug Prostatic Neoplasms medicine.disease medicine.anatomical_structure Endocrinology Oncology Cell culture Cancer cell Life Sciences & Biomedicine Pituitary Hormone-Releasing Hormones 1112 Oncology And Carcinogenesis Cell Division hormones hormone substitutes and hormone antagonists Research Article medicine.drug |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1990.236 |
| Popis: | We have investigated the possibility of a direct regulatory effect of gonadotrophin releasing hormone (GnRH) analogues on prostatic cancer cell growth. Here we report high affinity binding (Kd = 50 nM) of a GnRH analogue resulting in biphasic growth modulation of the human androgen-sensitive prostatic cancer cell line LNCaP. In contrast, the human androgen-insensitive prostatic cancer cell line DU145 showed low-affinity (Kd = 10 microM) binding without any biological response to the GnRH analogue. A GnRH-specific radioimmunoassay demonstrated GnRH-like immunoreactivity in the concentrated culture medium from both cell lines. Seventy-six human benign and malignant tumours were assayed following surgical resection. Nineteen of 22 (86%) malignant tumours and 49 of 54 (91%) benign tumours, exhibited high affinity GnRH-analogue binding. Fourteen of 19 (74%) malignant tumours and 17 of 49 (35%) benign tumours exhibiting high affinity binding contained GnRH-like immunoreactivity, suggesting that this system may be involved in prostatic epithelial cell growth in vivo. |
| Databáze: | OpenAIRE |
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