Mycobacterium marinum lipooligosaccharides are unique caryophyllose-containing cell wall glycolipids that inhibit tumor necrosis factor-α secretion in macrophages
| Autor: | Bernadette Coddeville, Laurent Kremer, Elisabeth Elass, Emmanuel Maes, Adeline Burguière, Yann Guérardel, Yoann Rombouts |
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| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Dynamique des interactions membranaires normales et pathologiques (DIMNP), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institute of Biological Chemistry (IBC Sinica), Academia Sinica, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Magnetic Resonance Spectroscopy Glycobiology and Extracellular Matrices MESH: Amino Acid Sequence MESH: Genome Bacterial Biochemistry Mass Spectrometry Cell Wall MESH: Monosaccharides Gene cluster Carbohydrate Conformation MESH: Glycolipids MESH: Carbohydrate Conformation MESH: Bacterial Proteins 0303 health sciences Monosaccharides Cell Differentiation [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] MESH: Chromatography Thin Layer Multigene Family Tumor necrosis factor alpha Sequence Analysis MESH: Carbohydrates MESH: Cell Differentiation Molecular Sequence Data Carbohydrates Virulence Biology MESH: Mycobacterium marinum Cell Line Bacterial genetics Microbiology 03 medical and health sciences MESH: Sequence Analysis Glycolipid MESH: Cell Wall Bacterial Proteins Glycosyltransferase Humans Secretion [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence Molecular Biology Mycobacterium marinum 030304 developmental biology MESH: Mass Spectrometry MESH: Humans MESH: Molecular Sequence Data Tumor Necrosis Factor-alpha 030306 microbiology MESH: Magnetic Resonance Spectroscopy Macrophages MESH: Macrophages Cell Biology biology.organism_classification MESH: Cell Line MESH: Tumor Necrosis Factor-alpha biology.protein MESH: Multigene Family Chromatography Thin Layer Glycolipids MESH: Lipopolysaccharides Genome Bacterial |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (31), pp.20975-20988. ⟨10.1074/jbc.M109.011429⟩ Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (31), pp.20975-88. ⟨10.1074/jbc.M109.011429⟩ Journal of Biological Chemistry, 2009, 284 (31), pp.20975-88. ⟨10.1074/jbc.M109.011429⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M109.011429⟩ |
| Popis: | International audience; Earlier studies have reported a role for lipooligosaccharides (LOSs) in sliding motility, biofilm formation, and infection of host macrophages in Mycobacterium marinum. Although a LOS biosynthetic gene cluster has recently been identified in this species, many structural features of the different LOSs (LOS-I-IV) are still unknown. This clearly hampers assessing the contribution of each LOS in mycobacterial virulence as well as structure-function-based studies of these important cell wall-associated glycolipids. In this study, we have identified an M. marinum isolate, M. marinum 7 (Mma7), which failed to produce LOS-IV but instead accumulated large amounts of LOS-III. Local genomic comparison of the LOS biosynthetic cluster established the presence of a highly disorganized region in Mma7 compared with the standard M strain, characterized by multiple genetic lesions that are likely to be responsible for the defect in LOS-IV production in Mma7. Our results indicate that the glycosyltransferase LosA alone is not sufficient to ensure LOS-IV biosynthesis. The availability of different M. marinum strains allowed us to determine the precise structure of individual LOSs through the combination of mass spectrometric and NMR techniques. In particular, we established the presence of two related 4-C-branched monosaccharides within LOS-II to IV sequences, of which one was never identified before. In addition, we provided evidence that LOSs are capable of inhibiting the secretion of tumor necrosis factor-alpha in lipopolysaccharide-stimulated human macrophages. This unexpected finding suggests that these cell wall-associated glycolipids represent key effectors capable of interfering with the establishment of a pro-inflammatory response. |
| Databáze: | OpenAIRE |
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