Expression of acyl-CoA-binding protein 5 from Rhodnius prolixus and its inhibition by RNA interference
| Autor: | Katia C. Gondim, Nils J. Færgeman, Muriel G. M. D. Almeida, Daniela S. Arêdes, David Majerowicz, Jens Knudsen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Oviposition Fat Body Gene Expression Biochemistry Fats RNA interference Animal Cells Hemolymph Gene expression Acyl-CoA-binding protein Medicine and Health Sciences Diazepam Binding Inhibitor Gene knockdown Multidisciplinary biology Chemistry Eukaryota Lipids Recombinant Proteins Cell biology Body Fluids Insects Ovaries Blood OVA Rhodnius Medicine Insect Proteins RNA Interference Anatomy Cellular Types Research Article Arthropoda Science 03 medical and health sciences Genetics Animals Rhodnius prolixus Gene Triglycerides 030102 biochemistry & molecular biology Organisms Reproductive System Biology and Life Sciences Proteins Lipid metabolism Cell Biology biology.organism_classification Lipid Metabolism Invertebrates 030104 developmental biology Germ Cells Gene Expression Regulation Oocytes Acyl Coenzyme A Carrier Proteins |
| Zdroj: | PLoS ONE Almeida, M G M D, Arêdes, D S, Majerowicz, D, Færgeman, N J, Knudsen, J & Gondim, K C 2020, ' Expression of acyl-CoA-binding protein 5 from Rhodnius prolixus and its inhibition by RNA interference ', PLOS ONE, vol. 15, no. 1, e0227685 . https://doi.org/10.1371/journal.pone.0227685 PLoS ONE, Vol 15, Iss 1, p e0227685 (2020) |
| ISSN: | 1932-6203 |
| Popis: | The acyl-CoA-binding proteins (ACBP) act by regulating the availability of acyl-CoA in the cytoplasm and must have essential functions in lipid metabolism. The genome of the kissing-bug Rhodnius prolixus encodes five proteins of this family, but little is known about them. In this study we investigated the expression and function of RpACBP-5. Feeding induced RpACBP-5 gene expression in the posterior midgut, and an increase of about four times was observed two days after the blood meal. However, the amount of protein, which was only detected in this organ, did not change during digestion. The RpACBP-5 gene was also highly expressed in pre-vitellogenic and vitellogenic oocytes. Recombinant RpACBP-5 was shown to bind to acyl-CoA of different lengths, and it exhibited nanomolar affinity to lauroyl-CoA in an isothermal titration assay, indicating that RpACBP-5 is a functional ACBP. RpACBP-5 knockdown by RNA interference did not affect digestion, egg laying and hatching, survival, or accumulation of triacylglycerol in the fat body and oocytes. Similarly, double knockdown of RpACBP-1 and RpACBP-5 did not alter egg laying and hatching, survival, accumulation of triacylglycerol in the fat body and oocytes, or the neutral lipid composition of the posterior midgut or hemolymph. These results show that RpACBP-5 is a functional ACBP but indicate that the lack of a detectable phenotype in the knockdown insects may be a consequence of functional overlap of the proteins of the ACBP family found in the insect. |
| Databáze: | OpenAIRE |
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