APETALA2 control of barley internode elongation
| Autor: | Sarah M. McKim, Mats Hansson, Michael Cummins, Rowan Meikle, Trisha Mcallister, Vrushali Patil, Jenny Morris, Pete E. Hedley, Ewan Mollison, Hannah I. McDermott, Christoph Dockter, Joana Clara Silva, Robbie Waugh |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Peduncle (anatomy)
Meristem Mutant Sequence Homology Flowers Biology Genes Plant 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Jasmonate Gene Expression Regulation Plant Cereal development Stem elongation Phase change Molecular Biology 030304 developmental biology Plant stem Homeodomain Proteins 0303 health sciences Methyl jasmonate Arabidopsis Proteins Gene Expression Regulation Developmental food and beverages Hordeum Plants Genetically Modified APETALA2 Cell biology chemistry Inflorescence Intercalary meristem Ectopic expression Gibberellin 030217 neurology & neurosurgery Research Article Developmental Biology |
| Zdroj: | Development (Cambridge, England) |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | Many plants dramatically elongate their stems during flowering, yet how this response is coordinated with the reproductive phase is unclear. We demonstrate that microRNA (miRNA) control of APETALA2 (AP2) is required for rapid, complete elongation of stem internodes in barley, especially of the final ‘peduncle’ internode directly underneath the inflorescence. Disrupted miR172 targeting of AP2 in the Zeo1.b barley mutant caused lower mitotic activity, delayed growth dynamics and premature lignification in the peduncle leading to fewer and shorter cells. Stage- and tissue-specific comparative transcriptomics between Zeo1.b and its parent cultivar showed reduced expression of proliferation-associated genes, ectopic expression of maturation-related genes and persistent, elevated expression of genes associated with jasmonate and stress responses. We further show that applying methyl jasmonate (MeJA) phenocopied the stem elongation of Zeo1.b, and that Zeo1.b itself was hypersensitive to inhibition by MeJA but less responsive to promotion by gibberellin. Taken together, we propose that miR172-mediated restriction of AP2 may modulate the jasmonate pathway to facilitate gibberellin-promoted stem growth during flowering. Summary: Regulation of reproductive stem elongation in barley by APETALA2 suggests a pivotal role for phase change repression of JA-associated responses to promote internode growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|