CpG Motif Identification for Veterinary and Laboratory Species Demonstrates That Sequence Recognition Is Highly Conserved
| Autor: | Lorne A. Babiuk, Sylvia van Drunen Littel-van den Hurk, Arthur M. Krieg, Robert Rankin, X. P. Ioannou, Reno Pontarollo, Rolf Hecker |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Veterinary Medicine Pharmacology Genetics Veterinary medicine Base Sequence medicine.drug_class Enzyme-Linked Immunosorbent Assay Biology Immunostimulant In vitro Immune system Oligodeoxyribonucleotides Species Specificity CpG site Inbred strain Infectious disease (medical specialty) Animals Laboratory Adjuvanticity medicine Animals CpG Islands Female Large animal |
| Zdroj: | Antisense and Nucleic Acid Drug Development. 11:333-340 |
| ISSN: | 1087-2906 |
| DOI: | 10.1089/108729001753231713 |
| Popis: | Oligodinucleotides containing CpG motifs stimulate vertebrate immune cells in vitro, have proven efficacy in murine disease models and are currently being tested in human clinical trials as therapies for cancer, allergy, and infectious disease. As there are no known immunostimulatory motifs for veterinary species, the potential of CpG DNA as a veterinary pharmaceutical has not been investigated. Here, optimal CpG motifs for seven veterinary and three laboratory species are described. The preferential recognition of a GTCGTT motif was strongly conserved across two vertebrate phyla, although a GACGTT motif was optimal for inbred strains of mice and rabbits. In a subsequent adjuvanticity trial, the in vitro screening methodology was validated in sheep, representing the first demonstration of CpG DNA efficacy in a veterinary species. These results should provide candidate immunostimulant and therapeutic drugs for veterinary use and enable the testing of CpG DNA in large animal models of human disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|