Genetic alterations within TLR genes in development of Toxoplasma gondii infection among Polish pregnant women
| Autor: | Dorota Nowakowska, Jan Wilczyński, Wioletta Wujcicka |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Linkage disequilibrium Adolescent Single-nucleotide polymorphism Polymorphism Single Nucleotide Young Adult 03 medical and health sciences 0302 clinical medicine Pregnancy parasitic diseases Genotype Humans Medicine Pregnancy Complications Infectious Allele Gene Genetics biology business.industry Haplotype Toxoplasma gondii General Medicine Prognosis biology.organism_classification Toll-Like Receptor 2 Toll-Like Receptor 4 TLR2 030104 developmental biology Case-Control Studies Toll-Like Receptor 9 Immunology Female business Toxoplasma Biomarkers Toxoplasmosis Follow-Up Studies 030215 immunology |
| Zdroj: | Advances in Medical Sciences. 62:216-222 |
| ISSN: | 1896-1126 |
| Popis: | Purpose The research was conducted to evaluate the role of genotypes, haplotypes and multiple-SNP variants in the range of TLR2 , TLR4 and TLR9 single nucleotide polymorphisms (SNPs) in the development of Toxoplasma gondii infection among Polish pregnant women. Material and methods The study was performed for 116 Polish pregnant women, including 51 patients infected with T. gondii , and 65 age-matched control pregnant individuals. Genotypes in TLR2 2258 G > A, TLR4 896 A > G, TLR4 1196 C > T and TLR9 2848 G > A SNPs were estimated by self-designed, nested PCR-RFLP assays. Randomly selected PCR products, representative for distinct genotypes in the studied polymorphisms, were confirmed by sequencing. All the genotypes were calculated for Hardy-Weinberg (H-W) equilibrium and TLR4 variants were tested for linkage disequilibrium. Relationships were assessed between alleles, genotypes, haplotypes or multiple-SNP variants in TLR polymorphisms and the occurrence of T. gondii infection in pregnant women, using a logistic regression model. Results All the analyzed genotypes preserved the H-W equilibrium among the studied groups of patients ( P > 0.050). Similar distribution of distinct alleles and individual genotypes in TLR SNPs, as well as of haplotypes in TLR4 polymorphisms, were observed in T. gondii infected and control uninfected pregnant women. However, the GACG multiple-SNP variant, within the range of all the four studied polymorphisms, was correlated with a decreased risk of the parasitic infection (OR 0.52, 95% CI 0.28-0.97; P ≤ 0.050). Conclusions The polymorphisms, located within TLR2 , TLR4 and TLR9 genes, may be involved together in occurrence of T. gondii infection among Polish pregnant women. |
| Databáze: | OpenAIRE |
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