The Microtubule-Associated Protein CLASP Sustains Cell Proliferation through a Brassinosteroid Signaling Negative Feedback Loop
| Autor: | Geoffrey O. Wasteneys, Mark F. Belmonte, Yuan Ruan, Laryssa Halat, Deirdre Khan, Chris Ambrose, Sylwia Jancowski |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell division Microtubule-associated protein Meristem Arabidopsis Down-Regulation Microtubules Plant Roots General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Microtubule Gene Expression Regulation Plant Brassinosteroids Sulfanilamides Brassinosteroid Cloning Molecular Cell Proliferation Brefeldin A biology Cell growth Arabidopsis Proteins food and beverages biology.organism_classification Cell biology Crosstalk (biology) Dinitrobenzenes 030104 developmental biology chemistry General Agricultural and Biological Sciences Microtubule-Associated Proteins Signal Transduction |
| Zdroj: | Current biology : CB. 28(17) |
| ISSN: | 1879-0445 |
| Popis: | The capacity for sustained cell division within the plant meristem is a critical determinant of organ structure and performance. This capacity is diminished in mutants lacking the microtubule-associated protein CLASP and when brassinosteroid signaling is increased. Here, we discovered that CLASP is both targeted by and promotes activity of the brassinosteroid pathway in Arabidopsis root apical meristems. We show that enhanced brassinosteroid signaling reduces CLASP transcript and protein levels, dramatically shifts microtubule organization, and reduces the number of cells in the meristem. In turn, CLASP, which tethers sorting nexin 1 vesicles to microtubules, sustains brassinosteroid signaling by fostering retrieval of endocytosed BRI1 receptors to the plasma membrane. clasp-1 null mutants have dampened brassinosteroid (BR)-mediated transcriptional activity and responses. Global transcript profiling confirmed the collapse of cell-cycle activity in clasp-1 and identified CLASP-mediated hormone crosstalk. Together, these findings reveal an unprecedented form of negative feedback supporting meristem homeostasis. |
| Databáze: | OpenAIRE |
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