Efficient mobilization of E1-deleted adenovirus type 5 vectors by wild-type adenoviruses of other serotypes
| Autor: | Martijn J. W. E. Rabelink, Mohamed A. Abou E. I. Hassan, Gijs A. Versteeg, Rob C. Hoeben, Hendrik J. Rademaker |
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| Rok vydání: | 2002 |
| Předmět: |
Adenoviruses
Human Genetic Vectors Wild type DNA replication Biology Cell Transformation Viral Virus Replication Virology Embryonic stem cell Cell Line Viral replication Cell culture Tumor Cells Cultured Adenovirus E1 Proteins Humans Luciferase Vector (molecular biology) Serotyping Gene Gene Deletion |
| Zdroj: | Journal of General Virology. 83:1311-1314 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-83-6-1311 |
| Popis: | Mobilization of replication-deficient adenovirus vectors can lead to spread and shedding of the vector. Here we show that in cultured HepG2 cells wild-type (wt) adenoviruses of subgroup A (Ad12), B (Ad7, 11 and 16), C (Ad1, 2 and 5) and E (Ad4) can efficiently mobilize Ad5CMVluc, a ΔE1ΔE3-Ad5 vector carrying the firefly luciferase gene as reporter. In addition, we show that Ad5CMVluc can be propagated on Ad12E1-transformed human embryonic retinoblasts. This provides evidence that expression of the E1 region of Ad12 is sufficient for mobilizing ΔE1-Ad5-derived vectors. Thus, in therapeutic applications of replication-defective Ad vectors any active Ad infection is of potential concern, independent of the serotype involved. To prevent vector mobilization by wt Ads, new vectors should be developed in which essential functions such as the initiation of DNA replication and genome packaging are restricted. |
| Databáze: | OpenAIRE |
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