Two-layer Electrospun System Enabling Wound Exudate Management and Visual Infection Response

Autor: Giuseppe Tronci, Mohamed Basel Bazbouz
Jazyk: angličtina
Rok vydání: 2019
Předmět:
bromothymol blue
Chronic wound
Two layer
Applied Physics (physics.app-ph)
02 engineering and technology
lcsh:Chemical technology
01 natural sciences
Biochemistry
Analytical Chemistry
fibres
chemistry.chemical_compound
Bromothymol blue
lcsh:TP1-1185
Instrumentation
Alanine
Biological Dressings
Wound.exudate
Physics - Applied Physics
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Atomic and Molecular Physics
and Optics

Electrospinning
colour change
free surface electrospinning
medicine.symptom
Swelling
0210 nano-technology
core-shell fibres
Exudate
FOS: Physical sciences
Color
010402 general chemistry
Article
Polymethacrylic Acids
Drug Resistance
Bacterial

medicine
Humans
Electrical and Electronic Engineering
Acrylic acid
Wound Healing
technology
industry
and agriculture

Water
Physics - Medical Physics
infection
0104 chemical sciences
chemistry
Bromthymol Blue
Wound Infection
Nanoparticles
Wounds and Injuries
Medical Physics (physics.med-ph)
Biomedical engineering
Zdroj: Sensors (Basel, Switzerland)
Sensors
Volume 19
Issue 5
Sensors, Vol 19, Iss 5, p 991 (2019)
ISSN: 1424-8220
Popis: The spread of antimicrobial resistance calls for chronic wound management devices that can engage with the wound exudate and signal infection by prompt visual effects. Here, the manufacture of a two-layer fibrous device with independently-controlled exudate management capability and visual infection responsivity was investigated by sequential free surface electrospinning of poly(methyl methacrylate-co-methacrylic acid) (PMMA-co-MAA) and poly(acrylic acid) (PAA). By selecting wound pH as infection indicator, PMMA-co-MAA fibres were encapsulated with halochromic bromothymol blue (BTB) to trigger colour changes at infection-induced alkaline pH. Likewise, the exudate management capability was integrated via the synthesis of a thermally-crosslinked network in electrospun PAA layer. PMMA-co-MAA fibres revealed high BTB loading efficiency (>
80 wt.%) and demonstrated prompt colour change and selective dye release at infected-like media (pH >
7). The synthesis of the thermally-crosslinked PAA network successfully enabled high water uptake (WU = 1291 ±
48 &minus
2369 ±
34 wt.%) and swelling index (SI = 272 ±
4 &minus
285 ±
3 a.%), in contrast to electrospun PAA controls. This dual device functionality was lost when the same building blocks were configured in a single-layer mesh of core-shell fibres, whereby significant BTB release (~70 wt.%) was measured even at acidic pH. This study therefore demonstrates how the fibrous configuration can be conveniently manipulated to trigger structure-induced functionalities critical to chronic wound management and monitoring.
Databáze: OpenAIRE