Relationship of large and small invertases in Saccharomyces: mutant selectively deficient in small invertase

Autor:	J O Lampen, T Mizunaga, B B Abrams, R A Hackel
Rok vydání:	1978
Předmět:	biology Strain (chemistry) Mutant Saccharomyces cerevisiae Methane sulfonate biology.organism_classification Microbiology Saccharomyces Sucrase Invertase Affinity chromatography Biochemistry Mutation Centrifugation Density Gradient Chromatography Gel Molecular Biology Research Article
Zdroj:	Journal of Bacteriology. 135:809-817
ISSN:	1098-5530 0021-9193
DOI:	10.1128/jb.135.3.809-817.1978
Popis:	A mutant strain of Saccharomyces cerevisiae (D10-ER1) has been isolated after a two-step mutagenesis of strain 4059-358D (SUC 1) using ethyl methane sulfonate. Cells of this new strain produced a level of total invertase equaling that of 4059 but contained only trace amounts of the small, internal, aglycan form of the enzyme (less than 0.1% of total in D10-ER1 compared with 6% in 4059). When D10.ER1 was crossed with an invertase-hyperproducing strain dgr3 (SUC3), progeny were isolated (HZ400-5A and HZ400-2C) in which levels of total invertase had at least quadrupled. The percentage of small invertase, however, remained insignificant. Levels of small invertase in strain HZ400-5A were determined by affinity chromatography on conconavalin A-Sepharose, gel permeation chromatography, and isopycnic centrifugation in CsCl. The large invertase of the SUC1 yeasts described here was found to contain a form apparently greater in size than the large invertase of the SUC2 strain FH4C; this probably reflects a higher content of carbohydrate. The overall results of this study do not support a direct structural relationship between large and small invertases. The implications on invertase biosynthesis and structure are discussed.
Databáze:	OpenAIRE
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