Development of diagnostic SCAR markers for Meloidogyne graminicola, M-oryzae, and M-salasi associated with irrigated rice fields in Americas
| Autor: | Maria Fátima Grossi De Sá, Vanessa S. Mattos, Cesar Bauer Gomes, Philippe Castagnone-Sereno, Regina M. D. G. Carneiro, Diana Fernandez, Karine Mulet, Juvenil Enrique Cares |
|---|---|
| Přispěvatelé: | Universidade de Brasília (UnB), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), VANESSA DA SILVA MATTOS, UNB, JUVENIL ENRIQUE CARES, UNB, CESAR BAUER GOMES, CPACT, DIANA FERNANDEZ, IRD, MARIA FATIMA GROSSI DE SA, Cenargen, REGINA MARIA DECHECHI G CARNEIRO, Cenargen, PHILIPPE CASTAGNONE-SERENO, INRA., KARINE MULET, CNRS |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Species complex Oryza Sativa [SDV]Life Sciences [q-bio] Plant Science Oryza 01 natural sciences 03 medical and health sciences Graminicola Nematóide Botany Tylenchoidea Animals Oryza sativa biology food and beverages Nematóide das galhas Interspecific competition 030108 mycology & parasitology biology.organism_classification RAPD Marcador Molecular Random Amplified Polymorphic DNA Technique [SDE]Environmental Sciences Paddy field Americas Agronomy and Crop Science Brazil 010606 plant biology & botany |
| Zdroj: | Plant Disease Plant Disease, American Phytopathological Society, 2019, 103 (1), pp.83-88. ⟨10.1094/PDIS-12-17-2015-RE⟩ Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 0191-2917 |
| DOI: | 10.1094/PDIS-12-17-2015-RE⟩ |
| Popis: | Root-knot nematodes (RKN) cause important production losses of rice (Oryza sativa L.) in the world. Together with Meloidogyne graminicola Golden and Birchfield 1965, M. oryzae Maas, Sanders and Dede, 1978 and M. salasi López, 1984 have been causing damages in irrigated rice fields in Central and South America. In addition, six other RKN species may occur in rice fields in other regions of the world. Correct identification of Meloidogyne spp. is difficult but essential for the management of rice RKNs. The objective of this study was to develop some species-specific molecular markers for the diagnosis of South American RKN rice-related species. Isozyme phenotypes indicated the occurrence of some RKN species in the Brazilian samples, namely M. graminicola, M. oryzae, M. javanica, and two cryptic species designated as Meloidogyne sp. 2 and Meloidogyne sp. 3. Random amplified polymorphic DNA (RAPD) analysis of 16 isolates revealed interspecific genetic polymorphism between Meloidogyne spp., but isolates belonging to the same species (i.e., sharing the same esterase phenotype) always clustered together, whatever the species considered. Specific SCAR markers of 230, 120, and 160 bp were developed for M. graminicola, M. oryzae, and M. salasi, respectively. These SCAR markers may be potential molecular tools for application in routine diagnostic procedures subject to their validation with other rice RKN field populations in the world. Made available in DSpace on 2019-01-07T23:50:25Z (GMT). No. of bitstreams: 1 CesarBauerpdis12172015re.pdf: 794039 bytes, checksum: 830120057aef2230c37cfa99698e16ce (MD5) Previous issue date: 2019-01-07 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|