Anatomically Restricted Synergistic Antiviral Activities of Innate and Adaptive Immune Cells in the Skin
| Autor: | Jonathan W. Yewdell, Barbara Molon, Javier G. Magadán, Jack R. Bennink, Barbara F. Ngudiankama, Stephanie S. Cush, Glennys V. Reynoso, Heather D. Hickman, Vincenzo Bronte, James S. Gibbs, Erica J. Rubin |
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| Jazyk: | angličtina |
| Předmět: |
Keratinocytes
Chemokine Cancer Research Immunology and Microbiology (all) Cell Adaptive Immunity CD8-Positive T-Lymphocytes Monocytes Mice Cell Movement Vaccinia Innate Antigens Ly Viral Microscopy biology reactive nitrogen species Antigens Ly Chemokines Ly6G antigen mouse Peroxynitrous Acid Viral Load Acquired immune system Reactive Nitrogen Species medicine.anatomical_structure Administration Skin Diseases Viral medicine.symptom Chemokines Inflammation Vaccinia virus Administration Cutaneous Microbiology Animals Epidermis Microscopy Fluorescence Multiphoton Peroxynitrous Acid Immunity Innate Molecular Biology Skin Diseases Virus Article Fluorescence Immune system Immunology and Microbiology(all) Virology medicine Antigens Ly6G antigen mouse Multiphoton Innate immune system Immunity Cutaneous Ly Immunology biology.protein Parasitology CD8 |
| Zdroj: | Cell Host & Microbe. (2):155-168 |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2013.01.004 |
| Popis: | Summary Despite extensive ex vivo investigation, the spatiotemporal organization of immune cells interacting with virus-infected cells in tissues remains uncertain. To address this, we used intravital multiphoton microscopy to visualize immune cell interactions with virus-infected cells following epicutaneous vaccinia virus (VV) infection of mice. VV infects keratinocytes in epidermal foci and numerous migratory dermal inflammatory monocytes that outlie the foci. We observed Ly6G + innate immune cells infiltrating and controlling foci, while CD8 + T cells remained on the periphery killing infected monocytes. Most antigen-specific CD8 + T cells in the skin did not interact with virus-infected cells. Blocking the generation of reactive nitrogen species relocated CD8 + T cells into foci, modestly reducing viral titers. Depletion of Ly6G + and CD8 + cells dramatically increased viral titers, consistent with their synergistic but spatially segregated viral clearance activities. These findings highlight previously unappreciated differences in the anatomic specialization of antiviral immune cell subsets. |
| Databáze: | OpenAIRE |
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