Comparative evaluation of the isotopes 86Rb and 51Cr in a humoral cytotoxicity system
| Autor: | Ph. Chollet, J.M. Bidet, R. Plagne, B. Sauvezie, Miss Christine Delossedat |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Lysis
Cell Survival Immunology chemistry.chemical_element Chromium Radioisotopes Rubidium Chromium Animals Humans Immunology and Allergy Cytotoxic T cell Lymphocytes Cytotoxicity Cells Cultured Antilymphocyte Serum Radioisotopes Chromatography Isotope Complement System Proteins Cytotoxicity Tests Immunologic In vitro chemistry Isotope Labeling Rabbits |
| Zdroj: | Journal of Immunological Methods. 6:23-29 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(74)90086-6 |
| Popis: | A comparative study of 86 Rb and 51 Cr in a humoral cytotoxic system has been performed. Lymphocytes obtained from normal donors were separated in a gradient centrifugation system and labelled in vitro with isotope. In each tube, 10 6 lymphocytes were exposed to the action of antilymphocyte serum, and the activity remaining in the cells was determined and compared with that in controls. The fraction of marker not released is much larger with chromium than with rubidium; 86 Rb release is very fast, and nearly complete after 30 min, as shown by comparison with physical lysis techniques. The ratios control/experiment are usually 2:1 with chromium and 4:1 or more with rubidium, despite higher spontaneous release of the latter due to passive diffusion through the cell wall. It appears that 86 Rb gives a more consistent and more sensitive method than 51 Cr. |
| Databáze: | OpenAIRE |
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