The effect of chronic long-term intermittent hypobaric hypoxia on bone mineral density in rats: role of nitric oxide
| Autor: | Muhittin O. Yaman, Nermin Yelmen, Habibe Genc, Gulcan Guntas Korkmaz, Yesari Karter, Metin Hallac, Ibrahim Guner, Gulderen Sahin, Remisa Gelisgen, Duygu Uzun, Gonul Simsek |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Bone density Bone metabolism Endocrinology Diabetes and Metabolism Clinical Biochemistry Osteoporosis Nitric Oxide Biochemistry Nitric oxide Bone remodeling Inorganic Chemistry chemistry.chemical_compound l-NAME Bone Density Internal medicine Bone mineral density medicine Animals Humans Enzyme Inhibitors Rats Wistar Hypoxia Bone mineral Intermittent hypoxia Biochemistry (medical) General Medicine medicine.disease Alkaline Phosphatase Rats Osteopenia Endocrinology NG-Nitroarginine Methyl Ester chemistry Parathyroid Hormone Hypobaric chamber Chronic Disease Cytokines |
| Zdroj: | Biological trace element research. 154(2) |
| ISSN: | 1559-0720 |
| Popis: | Intermittent hypoxia is the most common pattern of hypoxic exposure in humans. The effect of chronic long-term intermittent hypobaric hypoxia (CLTIHH) on bone metabolism is not investigated. We examined the effect of CLTIHH on bone metabolism and the role of nitric oxide (NO) in this process. The rats were divided into three groups in this study. The animals in groups I and II have been exposed to CLTIHH. The animals in group II were also treated with nitric oxide synthase inhibitor NG-nitro-l-arginine methyl ester. To obtain CLTIHH, rats were placed in a hypobaric chamber (430 mm Hg; 5 h/day, 5 days/week, 5 weeks). The group III (control) rats breathed room air in the same environment. At the begining of the experiments, bone mineral density (BMD) of the animals were measured, and blood samples were collected from the tail vein. After the 5-week CLTIHH period, the same measurements were repeated. Parathyroid hormone, calcium, phosphate, bone alkaline phosphatase (b-ALP), NO, interleukin 1 beta, interleukin 6, and tumor necrosis factor alpha levels were determined. The cytokines, NO levels, and BMD in CLTIHH-induced rats were higher compared with baseline and control values. The cytokines, b-ALP, and BMD increased while NO levels decreased in the group II compared with baseline values. BMD values of group II were lower than group I but higher than control group. Our results suggested that CLTIHH has positive effects on bone density. Intermittent hypoxia protocols may be developed for treatment and prevention of osteopenia and osteoporosis. © 2013 Springer Science+Business Media New York. |
| Databáze: | OpenAIRE |
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