UbcD4, an ortholog of E2-25K/Ube2K, is essential for activation of the immune deficiency pathway in Drosophila
| Autor: | Eun Sil Park, Yung Joon Yoo, Young-Joon Kim, Muthukumar Elangovan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biophysics Ubiquitin-conjugating enzyme Biochemistry 03 medical and health sciences Immune system Ubiquitin Animals Drosophila Proteins Molecular Biology chemistry.chemical_classification Gene knockdown biology Ubiquitination Cell Biology Immunity Innate Cell biology 030104 developmental biology Enzyme chemistry Ubiquitin-Conjugating Enzymes biology.protein Drosophila Tumor necrosis factor alpha Signal transduction Drosophila Protein Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 469:891-896 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2015.12.062 |
| Popis: | Ubiquitination is a key regulatory mechanism in the immune deficiency (IMD) pathway in Drosophila. In this study, we first developed a simple immunoblot method to identify components involved in this pathway. Considering the emerging roles of ubiquitin-conjugating enzymes (E2s) in determining ubiquitin chain types and ubiquitination speed, we screened for E2s required for IMD activation. We found that UbcD4, in addition to the previously reported E2s Effete and Bendless, was required for activation of the IMD pathway. RNAi-mediated knockdown of the UbcD4 ortholog, E2-25K/Ube2K, inhibited TNFα- and LPS-mediated activation of the NF-κB pathway, implying that UbcD4 and E2-25K/Ube2K play a conserved role as positive regulators in both pathways. |
| Databáze: | OpenAIRE |
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