A novel dual allosteric activation mechanism of Escherichia coli ADP-glucose pyrophosphorylase: the role of pyruvate
| Autor: | Mabel Cristina Aleanzi, Miguel A. Ballicora, Alberto A. Iglesias, Matías Damián Asención Diez |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
lcsh:Medicine
Glucose-1-Phosphate Adenylyltransferase medicine.disease_cause Biochemistry Substrate Specificity purl.org/becyt/ford/1 [https] chemistry.chemical_compound Fructosediphosphates Enzyme Chemistry lcsh:Science 2. Zero hunger chemistry.chemical_classification Multidisciplinary Glycogen Bioquímica y Biología Molecular Allosteric regulation Recombinant Proteins Enzymes Carbohydrate Metabolism CIENCIAS NATURALES Y EXACTAS Allosteric Site Research Article Bacteria glycogen Biology Ciencias Biológicas Enzyme Regulation Enzyme activator medicine Escherichia coli Enzyme kinetics Pyruvates purl.org/becyt/ford/1.6 [https] Enzyme Kinetics Activator (genetics) lcsh:R Synergic regulation Biology and Life Sciences Proteins Enzyme Activation Kinetics Enzyme Metabolism chemistry Enzymology Biocatalysis lcsh:Q ADP-glucose synthesis |
| Zdroj: | PLoS ONE, Vol 9, Iss 8, p e103888 (2014) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Fructose-1,6-bisphosphate activates ADP-glucose pyrophosphorylase and the synthesis of glycogen in Escherichia coli. Here, we show that although pyruvate is a weak activator by itself, it synergically enhances the fructose-1,6-bisphosphate activation. They increase the enzyme affinity for each other, and the combination increases Vmax, substrate apparent affinity, and decreases AMP inhibition. Our results indicate that there are two distinct interacting allosteric sites for activation. Hence, pyruvate modulates E. coli glycogen metabolism by orchestrating a functional network of allosteric regulators. We postulate that this novel dual activator mechanism increases the evolvability of ADP-glucose pyrophosphorylase and its related metabolic control. Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Loyola University Chicago. Department of Chemistry; Estados Unidos Fil: Aleanzi, Mabel C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina Fil: Ballicora, Miguel A.. Loyola University Chicago. Department of Chemistry; Estados Unidos |
| Databáze: | OpenAIRE |
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