Decalcification and staining of archaeological bones, with histochemical interpretation of metachromasia

Autor:	Helge Andersen, J. Balslev Jørgensen
Rok vydání:	1960
Předmět:	Bone decalcification Staining and Labeling Metachromasia Histological Techniques Paleontology Azure A Archaeology Bone and Bones Staining chemistry.chemical_compound chemistry Sodium citrate Paraffin section Toluidine Anatomy Citric acid Coloring Agents
Zdroj:	Stain technology. 35
ISSN:	0038-9153
Popis:	Metachromatic staining with azure A and toluidine blue was achieved on decalcified archaeological bones. The material was fixed in 10% formalin and in absolute alcohol and paraffin sections were stained in 0.1% azure A in 30% ethanol as well as in 0.5% aqueous toluidine blue. Various methods of decalcification are compared, and the authors recommend decalcification in equal parts of 2% citric acid and 20% sodium citrate, pH about 6.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cc6ea575a335c15b221a70b62268a2a https://pubmed.ncbi.nlm.nih.gov/13793310 Zobrazit plný text záznamu