Detection and identification of individual antigen molecules in human serum with pulsed semiconductor lasers

Autor:	Jürgen Wolfrum, C. Zander, B. Ullrich, K. H. Drexhage, Markus Sauer, Sepp Kaul, R. Müller
Rok vydání:	1997
Předmět:	Physics and Astronomy (miscellaneous) Chemistry General Engineering General Physics and Astronomy Nanotechnology Laser Fluorescence law.invention Semiconductor laser theory Full width at half maximum Antigen law Biophysics Molecule Diode
Zdroj:	Scopus-Elsevier
ISSN:	1432-0649 0946-2171
DOI:	10.1007/s003400050292
Popis:	The fluorescence bursts of individual antibody molecules BM-7 (IgG1) labeled with single dye molecules were detected and identified by the characteristic fluorescence lifetimes of the dyes Cy5 (1: 5n s) and JA169 (2: 7n s) directly in neat human serum. Fluorescence excitation was performed by a short-pulse (FWHM< 400 ps, 50 MHz) semiconductor diode laser. The tumor marker mucine (MUC1) was detect- ed in neat human serum by single-molecule events contain- ing both fluorescence lifetimes indicating specific binding of both antibody molecules (Cy5-BM-7 and JA169-BM-7). The sensitivity achieved allows the detection of antigens at concentrations below 10 11 M without separation steps.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cc2c8dc21effab5b9a11ed107968ad7 https://doi.org/10.1007/s003400050292 Zobrazit plný text záznamu Plný text ve formátu PDF