Application of FTA® Cards for detection and storage of avian influenza virus
| Autor: | Krzysztof Wyrostek, Michał Jóźwiak, Zenon Minta, Katarzyna Domańska-Blicharz, Krzysztof Śmietanka, Monika Olszewska-Tomczyk |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Veterinary medicine animal diseases 030106 microbiology Allantoic fluid avian influenza virus Biology fta® cards medicine.disease_cause storage 0403 veterinary science 03 medical and health sciences Immune system SF600-1100 medicine Infectivity Avian influenza virus General Veterinary virus diseases RNA 04 agricultural and veterinary sciences Pathogenicity Virology Influenza A virus subtype H5N1 Reverse transcription polymerase chain reaction molecular detection |
| Zdroj: | Journal of Veterinary Research, Vol 60, Iss 1, Pp 1-6 (2016) |
| ISSN: | 2450-8608 |
| Popis: | Introduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenicity H5N1 subtypes. Detection of viral material was conducted using molecular RT-PCR and rRT- PCR method. Results: The infectivity of LPAIV/H7N1 and HPAIV/H5N1 was completely inactivated within 1 h and 24 h after adsorption to FTA® Cards at room temperature, respectively. Viruses stored on FTA® Cards had detection limit approximately 1 log10 lower than live viruses. Viral RNA of both strains were detectable on the cards by rRT-PCR for a minimum of 150 d, irrespectively of storage temperatures (room temperature, -20ºC). RNA was also detected in all samples obtained from SPF chickens experimentally infected with HPAI/H5N1 on 3rd and 4th day post-infection (p.i.). Conclusion: FTA® Cards enable safe and effective alternative transport of samples for molecular diagnosis of AIV. |
| Databáze: | OpenAIRE |
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