Quantification of [123I]PE2I binding to dopamine transporters with SPET
| Autor: | Charlotte Videbæk, Claus Svarer, Lars H. Pinborg, Gitte M. Knudsen, Olaf B. Paulson, Stig Yndgaard |
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| Rok vydání: | 2002 |
| Předmět: |
Nortropanes
Nerve Tissue Proteins Models Biological Sensitivity and Specificity In vivo Humans Tissue Distribution Radiology Nuclear Medicine and imaging Dopamine transporter Cerebral Cortex Tomography Emission-Computed Single-Photon Volume of distribution Dopamine Plasma Membrane Transport Proteins Membrane Glycoproteins biology Phantoms Imaging Chemistry business.industry Area under the curve Membrane Transport Proteins General Medicine Middle Aged Ligand (biochemistry) Corpus Striatum Models Chemical Dopamine receptor Area Under Curve Cerebrovascular Circulation biology.protein Radiopharmaceuticals Nuclear medicine business Quantitative analysis (chemistry) Xenon Radioisotopes Blood sampling |
| Zdroj: | European Journal of Nuclear Medicine and Molecular Imaging. 29:623-631 |
| ISSN: | 1619-7089 1619-7070 |
| DOI: | 10.1007/s00259-001-0742-9 |
| Popis: | The iodinated cocaine derivative [(123)I]PE2I is a new selective ligand for in vivo studies of the dopamine transporter (DAT) with single-photon emission tomography (SPET). The aim of the present study was to describe a method for accurate quantification of binding data following a bolus injection of [(123)I]PE2I. Six healthy subjects (age 51+/-24 years) underwent xenon-133 SPET for quantification of regional CBF and [(123)I]PE2I SPET for quantification of DAT binding. rCBFs were within normal limits in all subjects. Fitting data to a two-tissue compartment model resulted in striatal K(1) values of 0.39+/-0.08 ml ml(-1) min(-1), equal to a first-pass extraction fraction of 0.72+/-0.13. Distribution volumes (DVs) were calculated using compartment analysis, area under the curve analysis and Logan analysis. Logan analysis is preferred since stable DV values were already obtained 120 min after [(123)I]PE2I injection. Mean striatal DV was 37.9+/-9.6 ml ml(-1) and mean occipital cortex DV was 5.5+/-0.7 ml ml(-1). In the absence of local pathology in a reference tissue, Logan analysis without blood sampling is an attractive method for accurate quantification of striatal [(123)I]PE2I binding. The distribution volume ratio (DVR) (6.6+/-1.4) was in good agreement with the DVR calculated with blood (6.7+/-1.4). |
| Databáze: | OpenAIRE |
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