Inhibition of miR-423-5p suppressed prostate cancer through targeting GRIM-19
| Autor: | Zhou-da Zheng, Minggen Yang, Zaixiong Shen, Hai-li Lin, Tianqi Lin, Jiangui Lin, Zongkai Zou, Hongjie Liu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine animal structures Down-Regulation Mice Nude Apoptosis Biology Flow cytometry Mice 03 medical and health sciences Prostate cancer 0302 clinical medicine Western blot Prostate Cell Line Tumor Genetics medicine Animals Humans NADH NADPH Oxidoreductases MTT assay Cell Proliferation Mice Inbred BALB C Gene knockdown medicine.diagnostic_test Cell growth Prostatic Neoplasms General Medicine medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Cancer research Female Apoptosis Regulatory Proteins |
| Zdroj: | Gene. 688:93-97 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2018.11.021 |
| Popis: | Objective To determine the effect of miR-423-5p on the progression of prostate cancer (PC). Methods miR-423-5p and GRIM-19 expressions were detected by qRT-PCR and western blot. PC cell proliferation was measured by MTT assay. PC cell apoptosis was detected by flow cytometry. Dual luciferase reporter assay was used to confirm the interaction between miR-423-5p and GRIM-19. Results Compared with normal prostate tissues and prostate epithelial cell HPrEC, miR-423-5p was up-regulated in human PC tissues and PC3 cells, whereas GRIM-19 expression was decreased. Inhibition of miR-423-5p suppressed PC3 cell proliferation, promoted PC3 cell apoptosis, and decreased anti-apoptosis protein BCL-2 expression. GRIM-19 was a target of miR-423-5p, and GRIM-19 was negatively regulated by miR-423-5p in PC3 cells. In addition, miR-423-5p knockdown inhibited the proliferation and promoted the apoptosis of PC3 cells through GRIM-19. In vivo experiments showed that miR-423-5p inhibitor administration reduced tumor volume, down-regulated miR-423-5p and GRIM-19 expressions in PC tissues of nude mice. Conclusion Inhibition of miR-423-5p suppressed PC through targeting GRIM-19. |
| Databáze: | OpenAIRE |
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