The preparation of stereospecific tritium-labeled reduced nicotinamide adenine dinucleotide phosphate
| Autor: | John A. Kepler, Clarence Cook, RI Freudenthal |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Isocitrates
Reduced nicotinamide-adenine dinucleotide Stereochemistry Radiochemistry Biophysics Stereoisomerism Borohydrides Cell Biology Tritium Phosphate Biochemistry Isocitrate Dehydrogenase chemistry.chemical_compound Stereospecificity chemistry Isotope Labeling Chromatography Gel Specific activity Chromatography Thin Layer Pyridine nucleotide Molecular Biology NADP |
| Zdroj: | Analytical Biochemistry. 51:632-636 |
| ISSN: | 0003-2697 |
| Popis: | A method has been developed for the preparation of a high specific activity stereospecifically labeled tritiated NADPH. In this procedure, tritium is enzymatically transferred from d-isocitric acid-2-3H (8 Ci/mmole) to the A face of a pyridine nucleotide during its stereospecific reduction, resulting in the formation of NADPH-4A-3H (2 Ci/mmole). |
| Databáze: | OpenAIRE |
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