A submicromolar assay for nonpolar acids in plasma and depot fat
| Autor: | Howard Ko, Max E. Royer |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Nitrogen
Depot Biophysics Hydroxybutyrates Oleic Acids Palmitic Acids Buffers Biochemistry Chemistry Techniques Analytical Palmitic acid Mice chemistry.chemical_compound Blood plasma Hydroxides Animals Pyruvates Molecular Biology Carbon Isotopes Heptane Chromatography Fatty Acids Extraction (chemistry) Cell Biology Sulfuric Acids Lactic acid Quaternary Ammonium Compounds Standard curve Adipose Tissue Linoleic Acids chemistry Lactates Solvents Indicators and Reagents Pyruvic acid |
| Zdroj: | Analytical Biochemistry. 20:205-214 |
| ISSN: | 0003-2697 |
| Popis: | A simple titrimetric assay, requiring about a third less time than that required for the Dole procedure (2), has been developed for determining submicromolar amounts of nonpolar acids (e.g., long-chain fatty acids) in depot fat and plasma. The method has the following capabilities: 1. 1. 0.03 and 0.06 μeq of added palmitic acid are detected with standard deviations of a single determination of 12–14 and 6–7%, respectively; these amounts of nonpolar acid correspond to those present in 0.1 and 0.2 ml of serum, respectively. 2. 2. About (95–97)% of added palmitic acid is recovered in the heptane phase by the extraction procedure. 3. 3. The common interfering materials in plasma, lactic acid, β-hydroxybutyric acid, and pyruvic acid gave positive interference to the extent on only about 0.3 to 0.5%, values which are about one-fourth the interference of the regular Dole extraction method. 4. 4. The presence or absence of Krebs-Ringer buffer or serum components has no significant effect on the recovery of palmitic acid by the modified method or by Dole's extraction method (1). Thus, standard curves for the esterified fatty acids may be constructed with or without the presence of these substances. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|