Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities
| Autor: | Redder, Esther, Kirschnick, Nils, Bobe, Stefanie, Hägerling, René, Hansmeier, Nils Rouven, Kiefer, Friedemann |
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| Přispěvatelé: | Universitäts- und Landesbibliothek Münster |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Imaging Techniques
610 Medicine and health Science Respiratory System Bioengineering Mouse Models Mice Transgenic Immunostaining Research and Analysis Methods Model Organisms Fluorescence Microscopy Genes Reporter Fluorescence Imaging Thoracic Diaphragm Medicine and Health Sciences Animals ddc:610 Lymphatic Vessels Genetically modified animals Mouse models Tissue clearing Light-sheet fluorescence microscopy Endothelial cells Fluorescence microscopy Thoracic diaphragm Staining Microscopy Microscopy Confocal Genetically Modified Animals Genetically Modified Organisms Biology and Life Sciences Light Microscopy Endothelial Cells Animal Models Vascular Endothelial Growth Factor Receptor-3 Luminescent Proteins Experimental Organism Systems Microscopy Fluorescence Specimen Preparation and Treatment Tissue Clearing Medicine and health Animal Studies Engineering and Technology Medicine Light-Sheet Fluorescence Microscopy Anatomy Genetic Engineering Research Article Biotechnology |
| Zdroj: | PLoS ONE, Vol 16, Iss 9, p e0249256 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Lymphatic vessels are indispensable for tissue fluid homeostasis, transport of solutes and dietary lipids and immune cell trafficking. In contrast to blood vessels, which are easily visible by their erythrocyte cargo, lymphatic vessels are not readily detected in the tissue context. Their invisibility interferes with the analysis of the three-dimensional lymph vessel structure in large tissue volumes and hampers dynamic intravital studies on lymphatic function and pathofunction. An approach to overcome these limitations are mouse models, which express transgenic fluorescent proteins under the control of tissue-specific promotor elements. We introduce here the BAC-transgenic mouse reporter strain Vegfr3-tdTomato that expresses a membrane-tagged version of tdTomato under control of Flt4 regulatory elements. Vegfr3-tdTomato mice inherited the reporter in a mendelian fashion and showed selective and stable fluorescence in the lymphatic vessels of multiple organs tested, including lung, kidney, heart, diaphragm, intestine, mesentery, liver and dermis. In this model, tdTomato expression was sufficient for direct visualisation of lymphatic vessels by epifluorescence microscopy. Furthermore, lymph vessels were readily visualized using a number of microscopic modalities including confocal laser scanning, light sheet fluorescence and two-photon microscopy. Due to the early onset of VEGFR-3 expression in venous embryonic vessels and the short maturation time of tdTomato, this reporter offers an interesting alternative to Prox1-promoter driven lymphatic reporter mice for instance to study the developmental differentiation of venous to lymphatic endothelial cells. Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster). |
| Databáze: | OpenAIRE |
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