Immobilization of digitoxin 12?-hydroxylase, a cytochrome P-450-dependent enzyme from cell cultures of Digitalis lanata EHRH
| Autor: | A. W. Alfermann, H. U. Seitz, E. Reinhard, Maike Petersen |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
Immobilized enzyme Cytochrome Digitoxin Substrate (chemistry) Plant Science General Medicine Biology biology.organism_classification Enzyme assay Enzyme chemistry Biochemistry Digitalis lanata biology.protein Microsome medicine Agronomy and Crop Science medicine.drug |
| Zdroj: | Plant Cell Reports. 6:200-203 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/bf00268479 |
| Popis: | Attempts were made to immobilize digitoxin 12β-hydroxylase, a membrane-bound, cytochrome P-450-dependent monooxygenase from cell cultures of Digitalis lanata. The optimum procedure was the entrapment of microsomes in 2% alginate by crosslinking the polysaccharide chains with CaCl2. After the immobilization of the enzyme about 70% of its activity was retained. The kinetic data such as the pH optimum and the optimum substrate concentrations were identical for the immobilized enzyme and freely suspended microsomes. Using β-methyldigitoxin as a substrate enzyme activity could be observed for more than 20 h. A continuous flow system for immobilized digitoxin 12β-hydroxylase is described. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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