Endometrial miRNome profile according to the receptivity status and implantation failure
| Autor: | Loubna Drissennek, Chloé Baron, Delphine Haouzi, Sophie Brouillet, Frida Entezami, Samir Hamamah |
|---|---|
| Přispěvatelé: | Développement embryonnaire précoce humain et pluripotence EmbryoPluripotency (UMR 1203), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-CHU Montpellier, Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Biologie du Cancer et de l'Infection (BCI ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Hôpital Américain de Paris, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Oncology
implantation window medicine.medical_specialty endometrial receptivity miscarriage Receptivity [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics Endometrium Miscarriage 03 medical and health sciences 0302 clinical medicine Pregnancy Internal medicine medicine Humans Embryo Implantation 030304 developmental biology Retrospective Studies 0303 health sciences 030219 obstetrics & reproductive medicine business.industry implantation failure Obstetrics and Gynecology Retrospective cohort study [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology General Medicine medicine.disease Embryo transfer 3. Good health Abortion Spontaneous MicroRNAs medicine.anatomical_structure Reproductive Medicine Cohort miRNAs Female business Live birth ART |
| Zdroj: | Human Fertility Human Fertility, Taylor & Francis, 2020, pp.1-13. ⟨10.1080/14647273.2020.1807065⟩ Human Fertility, 2020, pp.1-13. ⟨10.1080/14647273.2020.1807065⟩ |
| ISSN: | 1464-7273 |
| Popis: | International audience; This is a retrospective study to evaluate if the miRNome profile of endometrium samples collected during the implantation window predicts Assisted Reproduction Technology (ART) outcomes. We first investigated the endometrial miRNome profile according to the receptivity status in 20 patients with repeated implantation failures (RIF) (discovery cohort). After customized embryo transfer, the miRNome profiles of receptive patients with a positive or negative β-hCG, and with early miscarriage or live birth were analysed. Some differentially expressed miRNAs were selected for validation by RT-qPCR in endometrial samples from 103 RIF patients (validation cohort). Analysis of the different miRNome profiles identified endometrial receptivity, implantation failure, and early miscarriage-associated miRNA signatures that included 11, 261, and 76 miRNAs, respectively. However, only four miRNAs associated with the endometrial receptivity status (miR-455-3p and miR-4423-3p) and implantation failure (miR-152-3p and miR-155-5p) were significantly validated in endometrial samples. The miRNome profile of endometrial tissues during the implantation window can predict the pregnancy outcome. These data are crucial for opening new perspectives to predict implantation failure and consequently, to increase ART success. |
| Databáze: | OpenAIRE |
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