Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells
| Autor: | Ekaterine Tskitishvili, Kazuya Mimura, Takeshi Kanagawa, Hirotsugu Fukuda, Takuji Tomimatsu, Kumiko Temma-Asano, Yukiko Kinugasa-Taniguchi, Tadashi Kimura, Namuxila Sharentuya, Koichiro Shimoya |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Embryology medicine.medical_specialty Xanthine Oxidase Cell Survival Placenta Enzyme-Linked Immunosorbent Assay Receptors Cell Surface S100 Calcium Binding Protein beta Subunit Biology medicine.disease_cause Dinoprost Xanthine Andrology Tissue Culture Techniques Tissue culture Young Adult Pre-Eclampsia Fetal membrane Antigens CD Pregnancy Internal medicine Genetics medicine Humans Amnion Nerve Growth Factors Endothelial dysfunction Molecular Biology Cells Cultured Aldehydes S100 Proteins Endoglin Obstetrics and Gynecology Endothelial Cells Cell Biology medicine.disease Immunohistochemistry Endothelial stem cell Oxidative Stress Endocrinology medicine.anatomical_structure Reproductive Medicine Human umbilical vein endothelial cell Female Oxidative stress Developmental Biology |
| Zdroj: | Molecular human reproduction. 16(3) |
| ISSN: | 1460-2407 |
| Popis: | Oxidative stress with elevated intracellular Ca(2+) concentration as well as endothelial dysfunction is a component of pre-eclampsia. Our aim was to investigate the oxidative stress-dependent expression of Endoglin and Ca(2+)-binding S100B protein from villous and amniotic tissue cultures, and to assess sEng expression from S100B protein-stimulated endothelial cells. We initially examined Endoglin and Hydroxy-nonenal-(HNE)-modified proteins in the placentas and amnion obtained from women with pre-eclampsia (n = 8), and healthy controls (n = 8) by immunohistochemistry. To examine oxidative stress and the S100B protein effect on sEng expression from endothelial cells, normal villous and amniotic tissue cultures were stimulated by 4-HNE, sodium fluoride and xanthine/xanthine oxidase, whereas human umbilical vein endothelial cell cultures were treated with S100B protein in a dose- and time-dependent manner at 37 degrees C in an environment of 95% air and 5% of CO(2). Culture supernatants were assessed using ELISA. Cell viability was determined using MTS assay. The concentrations of sEng and S100B protein were significantly increased in the villous and amniotic tissue culture supernatants under oxidative stress. S100B protein-stimulated endothelial cells released sEng into conditioned media with a significantly higher expression levels at a concentration of 200 pM-20 nM S100B by 2 h, whereas treated with 200 nM of S100B endothelial cells significantly expressed sEng by 12 h and stimulated the cell proliferation by the same period of time. Our findings show that oxidative stress affects sEng and S100B protein expression from villous and amniotic tissues, and picomolar and low nanomolar concentrations of S100B protein significantly up-regulate sEng release from endothelial cells leading to endothelial dysfunction. |
| Databáze: | OpenAIRE |
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