Dietary Aloe Vera Gel Powder and Extract Inhibit Azoxymethane-induced Colorectal Aberrant Crypt Foci in Mice Fed a High-fat Diet
| Autor: | Shigeru Sonoda, Takeshi Chihara, Takashi Higashiguchi, Hidehiko Beppu, Takaaki Kaneko, Muneo Yamada, Kan Shimpo, Miyuki Tanaka, Fumiaki Abe |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Normal diet Epidemiology Blotting Western Azoxymethane Diet High-Fat Real-Time Polymerase Chain Reaction Aloe vera Mice chemistry.chemical_compound Insulin resistance Aberrant Crypt Foci Internal medicine medicine Animals Humans RNA Messenger Food science Aloe Glutathione Transferase chemistry.chemical_classification biology Plant Extracts Reverse Transcriptase Polymerase Chain Reaction Cell growth Public Health Environmental and Occupational Health Cytochrome P-450 CYP2E1 Glutathione medicine.disease biology.organism_classification Mice Inbred C57BL Enzyme Endocrinology Oncology chemistry Carcinogens Powders Colorectal Neoplasms Aberrant crypt foci |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 16:683-687 |
| ISSN: | 1513-7368 |
| DOI: | 10.7314/apjcp.2015.16.2.683 |
| Popis: | Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|