Antioxidant and lipoxygenase activities of polyphenol extracts from oat brans treated with polysaccharide degrading enzymes
Autor: | Nisita Ratnasari, Mallory E. Walters, Apollinaire Tsopmo |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Carbohydrase Cellulase Polysaccharide Article Food science 03 medical and health sciences Lipoxygenase chemistry.chemical_compound 0404 agricultural biotechnology Lipid oxidation medicine lcsh:Social sciences (General) lcsh:Science (General) chemistry.chemical_classification 030109 nutrition & dietetics Multidisciplinary biology Chemistry food and beverages 04 agricultural and veterinary sciences 040401 food science Biochemistry Polyphenol biology.protein lcsh:H1-99 Trolox lcsh:Q1-390 |
Zdroj: | Heliyon Heliyon, Vol 3, Iss 7 (2017) |
ISSN: | 2405-8440 |
DOI: | 10.1016/j.heliyon.2017.e00351 |
Popis: | This study used polysaccharide degrading enzymes and protein precipitation to extract polyphenols from oats and to determine their bioactivity. Duplicate oat brans were treated with viscozyme (Vis), cellulase (Cel) or no enzyme (control, CTL) then, proteins were removed in one set (Vis1, Cel1, CTL1) and not in the other (Vis2, Cel2, CTL2). HPLC analyses showed that for cellulase treated brans, precipitation of proteins increased phenolic acids and avenanthramides by 14%. Meanwhile, a decreased of 67% and 20% respectively was found for viscozyme and control brans. The effect of protein precipitation on soluble polyphenols is therefore dependent of the carbohydrase, as proteins with different compositions will interact differently with other molecules. Radical scavenging data showed that Cel1 and Vis1 had higher quenching effects on ROO• radicals with activities of 22.1 ± 0.8 and 23.5 ± 1.2 μM Trolox Equivalents/g defatted brans. Meanwhile, CTL2 had the highest HO• radicals inhibition (49.4 ± 2.8%) compared to 10.8–32.3% for others. Samples that highly inhibited lipoxygenase (LOX), an enzyme involved in lipid oxidation were Cel1 (23.4 ± 2.3%) and CTL1 (18 ± 0.4%). |
Databáze: | OpenAIRE |
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