Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Autor: Julián Mejía Morales, Tomaso Zambelli, Oana Dobre, Ines Lüchtefeld, Massimo Vassalli, Alice Bartolozzi, Michele Basso
Rok vydání: 2020
Předmět:
Lipid Bilayers
Pharmaceutical Science
Medicine (miscellaneous)
Modulus
02 engineering and technology
Applied Microbiology and Biotechnology
Nanoindentation
Indentation
Nanotechnology
Cytoskeleton
Physics
0303 health sciences
Stiffness
Brain
Cell mechanics
021001 nanoscience & nanotechnology
Force spectroscopy
Scanning probe microscopy
Force spectroscopy
Cell mechanics
Nanoindentation
Cytoskeleton
Actin cortex
Actin Cytoskeleton
lcsh:R855-855.5
Scanning probe microscopy
Molecular Medicine
medicine.symptom
Single-Cell Analysis
0210 nano-technology
Biological system
lcsh:Medical technology
Actin cortex
lcsh:Biotechnology
Biomedical Engineering
Bioengineering
Models
Biological
Cell Line
03 medical and health sciences
lcsh:TP248.13-248.65
Elastic Modulus
medicine
Humans
Elasticity (economics)
Actin
030304 developmental biology
Spectrum Analysis
Research
Actins
Elasticity
Kinetics
Stress
Mechanical
Zdroj: Journal of Nanobiotechnology
Journal of Nanobiotechnology, 18 (1)
Journal of Nanobiotechnology, Vol 18, Iss 1, Pp 1-11 (2020)
ISSN: 1477-3155
Popis: Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young’s modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young’s modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.
Journal of Nanobiotechnology, 18 (1)
ISSN:1477-3155
Databáze: OpenAIRE
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