Preliminary results of random amplification of polymorphic DNA among Triatominae of the phyllosoma complex (Hemiptera, Reduviidae)
Autor: | Simone Frédérique Brenière, Bruno Taveira, Ali Ouaissi, Janine M. Ramsey, Rosalinda Ordoñez, Marie-France Bosseno, Felipe Lozano-Kasten, Ezequiel Magallón-Gastélum |
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Rok vydání: | 2003 |
Předmět: |
Genetic Markers
Male Microbiology (medical) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 lcsh:QR1-502 Zoology random amplification of polymorphic DNA phylogeny COMPLEX D'ESPECES lcsh:Microbiology Phyllosoma Phylogenetics Triatoma infestans ETUDE COMPARATIVE Animals PHYLOGENIE MARQUEUR MOLECULAIRE Mexico Neighbor joining Triatominae Genetics biology VECTEUR TECHNIQUE RAPD biology.organism_classification Hemiptera Insect Vectors Random Amplified Polymorphic DNA Technique RAPD Reduviidae ELECTROPHORESE MALADIE DE CHAGAS TAXONOMIE BIOCHIMIQUE Female ANALYSE GENETIQUE phyllosoma complex |
Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 98, Iss 8, Pp 1033-1038 (2003) Memórias do Instituto Oswaldo Cruz, Volume: 98, Issue: 8, Pages: 1033-1038, Published: DEC 2003 |
ISSN: | 0074-0276 |
Popis: | In Mexico, Triatoma longipennis (Usinger), Triatoma picturata (Usinger), and Triatoma pallidipennis (Stal), primary Chagas disease vector species of the phyllosoma complex, were analyzed by randomly amplified polymorphic DNA (RAPD). Sixteen decametric primers resolved individual profiles not identical, but partially discriminative between species. Analysis based on pairwise presence/absence comparisons between the three species was performed using three primers and two outgroup species Triatoma infestans (Klug) and Triatoma barberi (Usinger). Fifty-three bands in total were scored, although only two bands were constant among the three phyllosoma complex species. Two other bands were constant only for T. longipennis and T. picturata together, and not present in T. pallidipennis. Neighbor Joining tree and the multiple correspondence analysis discriminated T. pallidipennis clearly from the other two species, although there was overlap between T. longipennis and T. picturata. The results indicate a close relationship between the studied species and support the hypothesis of their recent evolution. The suitability of RAPD to discern populations within the species is discussed. |
Databáze: | OpenAIRE |
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