Arabidopsis glucosinolates trigger a contrasting transcriptomic response in a generalist and a specialist herbivore

Autor: Heiko Vogel, Hanna M. Heidel-Fischer, Philippe Reymond, Fabian Schweizer
Rok vydání: 2017
Předmět:
0301 basic medicine
Glucosinolates
Arabidopsis
Down-Regulation
Moths
Generalist and specialist species
Biochemistry
Transcriptome
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Botany
Arabidopsis thaliana
Animals
Herbivory
Molecular Biology
Pieris brassicae
biology
Heliothis virescens
Sequence Analysis
RNA
Gene Expression Profiling
fungi
food and beverages
biology.organism_classification
Butterflies/drug effects
Butterflies/genetics
Butterflies/metabolism
Glucosinolates/pharmacology
Inactivation
Metabolic/genetics
Insect Proteins/metabolism
Larva/drug effects
Moths/drug effects
Moths/genetics
Moths/metabolism
Transcriptome/drug effects
Detoxification
Insect transcriptome
030104 developmental biology
chemistry
Insect Science
Glucosinolate
Larva
Inactivation
Metabolic
Insect Proteins
Adaptation
Butterflies
Zdroj: Insect biochemistry and molecular biology, vol. 85, pp. 21-31
ISSN: 1879-0240
Popis: Phytophagous insects have to deal with toxic defense compounds from their host plants. Although it is known that insects have evolved genes and mechanisms to detoxify plant allochemicals, how specialist and generalist precisely respond to specific secondary metabolites at the molecular level is less understood. Here we studied the larval performance and transcriptome of the generalist moth Heliothis virescens and the specialist butterfly Pieris brassicae feeding on Arabidopsis thaliana genotypes with different glucosinolate (GS) levels. H. virescens larvae gained significantly more weight on the GS-deficient mutant quadGS compared to wild-type (Col-0) plants. On the contrary, P. brassicae was unaffected by the presence of GS and performed equally well on both genotypes. Strikingly, there was a considerable differential gene expression in H. virescens larvae feeding on Col-0 compared to quadGS. In contrast, compared to H. virescens, P. brassicae displayed a much-reduced transcriptional activation when fed on both plant genotypes. Transcripts coding for putative detoxification enzymes were significantly upregulated in H. virescens, along with digestive enzymes and transposable elements. These data provide an unprecedented view on transcriptional changes that are specifically activated by GS and illustrate differential molecular responses that are linked to adaptation to diet in lepidopteran herbivores.
Databáze: OpenAIRE
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