Differential response to heat stress in outer and inner onion bulb scales
| Autor: | Adi Doron-Faigenboim, Ortal Galsurker, Amnon Lers, Avinoam Daus, Paula Teper-Bamnolker, Dani Eshel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology oxidation Plant Science 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Cell Wall Onions Browning Curing Heat shock biology Chemistry heat treatment fungi onion scale food and beverages Lipid metabolism heat tolerance defense response Lipid Metabolism Research Papers tissue browning 030104 developmental biology biology.protein Biophysics Osmoprotectant Plant–Environment Interactions Quercetin Desiccation Oxidation-Reduction Heat-Shock Response 010606 plant biology & botany Peroxidase |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Differential gene expression in the outer and inner scales of onion bulbs in response to heat stress accounts for the fact that only outer scales can form a protective dry brown skin. The formation of brown protective skin in onion bulbs can be induced by rapid post-harvest heat treatment. Onions that are peeled to different depths and are exposed to heat stress show that only the outer scales form the dry brown skin, whereas the inner scales maintain high water content and do not change color. Our study demonstrates that browning of the outer scale during heat treatment is due to an enzymatic process that is associated with high levels of oxidation components, such as peroxidase and quercetin glucoside. De novo transcriptome analysis revealed differential molecular responses of the outer and inner scales to heat stress. Genes involved in lipid metabolism, oxidation pathways, and cell-wall modification were highly expressed in the outer scale during heating. Defense response-related genes such as those encoding heat-shock proteins, antioxidative stress defense, or production of osmoprotectant metabolites were mostly induced in the inner scale in response to heat exposure. These transcriptomic data led to a conceptual model that suggests sequential processes for the development of browning and desiccation of the outer scale versus processes associated with defense response and heat tolerance in the inner scales. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|