Involvement of ZFPIP/Zfp462 in chromatin integrity and survival of P19 pluripotent cells

Autor: Stéphane Deschamps, Audrey Laurent, Daniel Guerrier, Barbara Nicol, Isabelle Pellerin, Julie Masse
Přispěvatelé: Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), De Villemeur, Hervé
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Cell division
MESH: 3T3 Cells
Cellular differentiation
Apoptosis
Mice
0302 clinical medicine
Chlorocebus aethiops
MESH: RNA
Small Interfering
MESH: Animals
MESH: Nerve Tissue Proteins
RNA
Small Interfering
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Cells
Cultured
Zinc finger
chromatin structure
0303 health sciences
3T3 Cells
differentiation
MESH: Gene Expression Regulation
Chromatin
Cell biology
DNA-Binding Proteins
MESH: COS Cells
P19 cell
medicine.anatomical_structure
MESH: Cell Survival
COS Cells
MESH: Cell Division
MESH: Pluripotent Stem Cells
Cell Division
MESH: Cells
Cultured
Pluripotent Stem Cells
zinc finger protein
Cell Survival
proliferation
Nerve Tissue Proteins
MESH: Carrier Proteins
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
3T3 cells
MESH: Chromosomal Instability
MESH: Chromatin
03 medical and health sciences
Chromosomal Instability
[SDV.BDD] Life Sciences [q-bio]/Development Biology
medicine
Animals
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
MESH: Mice
030304 developmental biology
Cell growth
MESH: Apoptosis
MESH: Embryo
Mammalian
Cell Biology
Embryo
Mammalian
Embryonic stem cell
MESH: Cercopithecus aethiops
Gene Expression Regulation
Carrier Proteins
030217 neurology & neurosurgery
Zdroj: Experimental Cell Research
Experimental Cell Research, Elsevier, 2010, 316 (7), pp.1190-201. ⟨10.1016/j.yexcr.2010.02.024⟩
Experimental Cell Research, 2010, 316 (7), pp.1190-201. ⟨10.1016/j.yexcr.2010.02.024⟩
ISSN: 0014-4827
1090-2422
DOI: 10.1016/j.yexcr.2010.02.024⟩
Popis: International audience; Toti- or pluripotent cells proliferation and/or differentiation have been shown to be strongly related to nuclear chromatin organization and structure over the last past years. We have recently identified ZFPIP/Zfp462 as a zinc finger nuclear factor necessary for correct cell division during early embryonic developmental steps of vertebrates. We thus questioned whether this factor was playing a general role during cell division or if it was somehow involved in embryonic cell fate or differentiation. To achieve this goal, we performed a knock-down experiment in the pluripotent P19 and differentiated 3T3 cell lines, both expressing endogenous ZFPIP/Zfp462. Using specific shRNA directed against ZFPIP/Zfp462 transcripts, we demonstrated that depletion of this protein induced cell death in P19 but had no effect in 3T3 cells. In addition, in the absence of the protein, the P19 cells exhibited a complete destructuration of pericentromeric domains associated with a redistribution of the HP1alpha proteins and an increase in DNA satellites transcribed RNAs level. These data suggested an instrumental role of ZFPIP/Zfp462 in maintaining the chromatin structure of pluripotent cells.
Databáze: OpenAIRE
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