Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease
| Autor: | Jordi Gomez-i-Prat, Lídia Gual-Gonzalez, Juliana Esperalba, Inés Oliveira-Souto, Tomás Pumarola, Israel Molina, Candela Fernández-Naval, Elena Sulleiro, Adrián Sánchez-Montalvá, Núria Serre-Delcor, Aroa Silgado, Lidia Goterris |
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| Přispěvatelé: | Institut Català de la Salut, [Silgado A, Goterris L, Esperalba J, Fernández-Naval C, Pumarola T, Sulleiro E] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. PROSICS Barcelona, Barcelona, Spain. [Gual-Gonzalez L] Laboratory of Vector-Borne and Zoonotic Diseases, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States. [Sánchez-Montalvá A, Oliveira-Souto I, Serre-Delcor N, Gomez-I-Prat J, Molina I] Servei de Malalties Infeccioses, Drassanes - Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. PROSICS Barcelona, Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Chagas disease
Microbiology (medical) medicine.medical_specialty Trypanosoma cruzi Serodiagnòstic Immunology Chronic Chagas' disease Otros calificadores::/diagnóstico [Otros calificadores] Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Gastroenterology Microbiology Serology enfermedades parasitarias::infecciones por protozoos::infecciones por Euglenozoa::tripanosomiasis::enfermedad de Chagas [ENFERMEDADES] Cellular and Infection Microbiology Internal medicine parasitic diseases medicine Other subheadings::/diagnosis [Other subheadings] Humans Chagas Disease Sampling (medicine) Retrospective Studies Original Research serological screening Rapid diagnostic test biology dried blood spot (DBS) Diagnostic Tests Routine business.industry Chagas Malaltia de - Diagnòstic Sang - Anàlisi Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Tests Routine [ANALYTICAL DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT] biology.organism_classification medicine.disease diagnóstico::técnicas y procedimientos diagnósticos::pruebas diagnósticas rutinarias [TÉCNICAS Y EQUIPOS ANALÍTICOS DIAGNÓSTICOS Y TERAPÉUTICOS] QR1-502 Dried blood spot diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::pruebas de bioquímica clínica::análisis químico de la sangre::análisis de manchas de sangre seca [TÉCNICAS Y EQUIPOS ANALÍTICOS DIAGNÓSTICOS Y TERAPÉUTICOS] Infectious Diseases biology.protein rapid diagnostic test (RDT) Antibody business community strategies Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Clinical Chemistry Tests::Blood Chemical Analysis::Dried Blood Spot Testing [ANALYTICAL DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT] Parasitic Diseases::Protozoan Infections::Euglenozoa Infections::Trypanosomiasis::Chagas Disease [DISEASES] |
| Zdroj: | Scientia Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) Frontiers in Cellular and Infection Microbiology |
| ISSN: | 2235-2988 |
| DOI: | 10.3389/fcimb.2021.736630 |
| Popis: | Trypanosoma cruzi; Mancha de sangre seca (DBS); Cribado serológico Trypanosoma cruzi; Taca de sang seca (DBS); Cribratge serològic Trypanosoma cruzi; Dried blood spot (DBS); Serological screening Background: Chagas disease is a public health problem not only in Latin America, but also in other regions, including Spain, due to migration movements. Conventional serological diagnosis requires an invasive sample (plasma or serum) and a well-equipped laboratory. To circumvent those limitations, blood samples dried on filter paper (DBS) or Rapid Diagnostic Test (RDT) could be a practical alternative to reference protocol for serological screening in epidemiological studies. We evaluated the usefulness of dried blood sampling and a rapid diagnostic test (Trypanosoma Detect™) for the detection of antibodies against T. cruzi for their use in community-based screening. Methodology/Principal Findings: A total of 162 stored paired whole-blood and serum samples from Latin American migrants and 25 negative-control blood samples were included. Diagnosis of chronic Chagas disease was performed in serum according to WHO algorithms. Blood samples were retrospectively collected as dried spots and then analyzed using two different serological techniques, enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (E-CLIA). Whole-blood samples were also used to evaluate a rapid diagnostic test based on immunochromatography. A better correlation with conventional serum was observed in dried blood elutes using E-CLIA than ELISA (97% vs. 77% sensitivity, respectively). Both assays reported 100% specificity. The median cut-off index values of E-CLIA for dried blood were significantly lower than those for serum (138.1 vs. 243.3, P |
| Databáze: | OpenAIRE |
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