Membrane Oxidative Damage Induced by Ionizing Radiation Detected by Diphenylhexatriene Fluorescence Lifetime Distributions
| Autor: | O. Sapora, G. Ravagnan, Tiziana Parasassi, Enrico Gratton |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Diphenylhexatriene
Radiological and Ultrasound Technology Bilayer technology industry and agriculture Phospholipid Analytical chemistry chemistry.chemical_element Dose-Response Relationship Radiation Dielectric Fluorescence Oxygen Ionizing radiation chemistry.chemical_compound Membrane chemistry Liposomes Biophysics lipids (amino acids peptides and proteins) Radiology Nuclear Medicine and imaging Oxidation-Reduction Phospholipids |
| Zdroj: | Parasassi, T; Ravagnan, G; Sapora, O; & Gratton, E. (1992). Membrane oxidative damage induced by ionizing radiation detected by diphenylhexatriene fluorescence lifetime distributions. International Journal of Radiation Biology, 61(6), 791-796. doi: 10.1080/09553009214551661. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/68k9r414 International journal of radiation biology 61 (1992): 791–796. doi:10.1080/09553009214551661 info:cnr-pdr/source/autori:Parasassi T, Ravagnan G, Sapora O, Gratton E/titolo:Membrane oxidative damage induced by ionizing radiation detected by diphenylhexatriene fluorescence lifetime distributions/doi:10.1080%2F09553009214551661/rivista:International journal of radiation biology (Print)/anno:1992/pagina_da:791/pagina_a:796/intervallo_pagine:791–796/volume:61 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/09553009214551661 |
| Popis: | The sensitivity of the fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) to the dielectric constant of its environment has been used to detect oxidative damage to phospholipid membranes induced by ionizing radiation. The DPH fluorescence decay in phospholipid vesicles is described well by a continuous distribution of lifetime values, reflecting the various DPH depths in the bilayer and related to the gradient of the dielectric constant. Ionizing radiation oxidizes unsaturated acyl residues of phospholipids, altering the dielectric constant across the bilayer, sharpening the distribution of DPH lifetimes and increasing the centre of the distribution. Ionizing radiation doses between 22 and 110 Gy were used, and were effective only in the presence of oxygen. A model based on the formation of packing defects in the bilayer describes the phenomenon. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| Databáze: | OpenAIRE |
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