Comparison of 5-fluorouracil-related gene expression levels between adenocarcinomas and squamous cell carcinomas of the lung
| Autor: | Yoko Karube, Hideo Umezu, Nobuhide Masawa, Motohiko Tamura, Osamu Araki, Koichi Honma, Norio Seki, Hiromi Ishihama, Shinichiro Miyoshi, Masayuki Chida |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Cancer Research Antimetabolites Antineoplastic Lung Neoplasms Orotate Phosphoribosyltransferase Adenocarcinoma Thymidylate synthase Tegafur Gene Expression Regulation Enzymologic Carcinoma medicine Dihydropyrimidine dehydrogenase Humans Radiology Nuclear Medicine and imaging Thymidine phosphorylase Lung cancer Dihydrouracil Dehydrogenase (NADP) Aged Neoplasm Staging Thymidine Phosphorylase biology business.industry General Medicine Thymidylate Synthase medicine.disease Prognosis Gene Expression Regulation Neoplastic Oncology Fluorouracil biology.protein Cancer research Carcinoma Squamous Cell Female business medicine.drug |
| Zdroj: | Japanese journal of clinical oncology. 39(1) |
| ISSN: | 1465-3621 |
| Popis: | BACKGROUND A recent meta-analysis study showed that post-operative adjuvant chemotherapy with UFT, an oral combination drug composed of tegafur [prodrug of 5-fluorouracil (5-FU)] and uracil [inhibitor of dihydropyrimidine dehydrogenase (DPD)] was associated with improved survival in patients with lung adenocarcinomas, but not in those with lung squamous cell carcinomas. METHODS We investigated the 5-FU-related gene expression levels of thymidylate synthase (TS), DPD, thymidine phosphorylase (TP) and orotate phosphoribosyl transferase (OPRT) in resected tumor specimens from 51 patients with adenocarcinomas and 47 with squamous cell carcinomas using quantitative reverse transcription-PCR, and compared those levels between the two histological types. RESULTS The relative gene expression values of TS, TP and OPRT were significantly lower in adenocarcinomas compared with squamous cell carcinomas, 1.60 +/- 0.86 versus 4.33 +/- 3.40 (P < 0.001), 0.84 +/- 0.52 versus 2.27 +/- 1.16 (P = 0.006) and 9.59 +/- 6.30 versus 16.94 +/- 12.04 (P < 0.001), respectively. The relative gene expression value of DPD was significantly greater in adenocarcinomas than those in squamous cell carcinomas, 2.33 +/- 1.22 versus 1.50 +/- 1.20 (P = 0.01). Lower expressions of TS and TP were observed more in adenocarcinomas (89.8%) than in squamous cell carcinomas (48.9%) (P < 0.001). CONCLUSION These data may explain that post-operative adjuvant chemotherapy with UFT was associated with improved survival in stage I patients with adenocarcinoma, but less with squamous cell carcinoma. |
| Databáze: | OpenAIRE |
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